Cellectra™ Human CD14 NanoSort Microbeads, Research grade

Application Data

Superior Cell Separation Performance (Comparison with Company M)
Separation performance was evaluated using samples containing 1×10⁷ human PBMCs, processed with CD14 microbeads from Acro, Company M at recommended dosages. Positive and negative fractions were collected, and 1×10⁶ cells from each fraction were immunostained with PE-conjugated anti-human CD14 antibody and 7-AAD, followed by flow cytometric analysis. Data indicated that Acro microbeads achieved a final purity of 98.01% (baseline: 22.03%), with a target cell residue of only 0.40% in the negative fraction. These findings validate outstanding separation performance, confirming an optimal balance of purity and recovery relative to Company M benchmarks.

Comparison of Cell Separation Performances
Isolation efficacy was validated using samples containing 1×10⁷ human PBMCs processed with the recommended dosage of Cellectra™ Human CD14 NanoSort Microbeads, in parallel with Company M. The comparative analysis demonstrated that Acro NanoSort Microbeads is consistent with Company M. Furthermore, high post-isolation cell viability was preserved, confirming suitability for sensitive downstream applications.

Cell Separation Performances after Freeze-Thawing and Accelerated Aging at 37℃
Freeze-thaw stability was verified by subjecting the microbeads to 2–3 cycles prior to separation testing. Comparative analysis against the standard batch confirmed that purity and cell recovery were maintained at baseline specifications, demonstrating robust stability. To ensure prolonged shelf life, storage at -20°C or below is recommended upon receipt (however, repeated freeze-thaw cycles are not advised for routine operational use).
Accelerated aging studies were conducted at 37°C to evaluate long-term stability profiles. Following 4 and 7-day incubation periods, the microbeads sustained high purity and cell recovery performance during PBMC separation. These data substantiate product stability and validate the established shelf-life specifications.