HEK293/Human EGF R Stable Cell Line

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CHEK-ATP148-2Vials (1Vial X 2)
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CHEK-ATP148
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  • Product Details
  • Performance Data
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  • Important Statement
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Advantages
  • Genetically modified cell lines best reflect MOA (Mechanism of Action)
  • Higher activity and larger assay window for robust and reproducible cell-based bioassay
  • Comprehensive application data to support assay development and validation
  • Full tracible record, stringent quality control and validated cell passage stability
  • Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
  • Global commercial license assistance whenever regulatory filing is required
Description

The HEK293/Human EGF R Stable Cell Line was engineered to express the receptor full length human EGF R (Uniprot: P00533-1), used to mimic cancer target cells. Surface expression of human EGF R was confirmed by flow cytometry.

Application

• Useful for cell-based EGF R binding assay.

Growth Properties

Adherent

Selection Marker

Puromycin (2 μg/mL)

Complete Growth Medium

DMEM medium + 10% FBS

Freeze Medium

Serum-free cell cryopreservation medium

Quantity

1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium

Storage

Frozen in liquid nitrogen.

Mycoplasma Testing

Negative

Sterility Testing

Negative

Instructions for Use

See data sheet for detailed culturing and assay protocol.