Human EGF R & (SRE-Luc) HEK293 Reporter Cell

Expression analysis of human EGF R on Human EGF R & (SRE-Luc) HEK293 Reporter Cell by FACS.
Cell surface staining was performed on Human EGF R & (SRE-Luc) HEK293 Reporter Cell or negative control cell using PE-labeled anti-EGF R antibody.

Inhibition of human EGF protein-induced reporter activity by anti-human EGFR-MET bispecific antibody.
This reporter cell was incubated with serial dilutions of antibodies in the presence of human EGF protein (Cat. No. EGF-H52H3) with a final concentration of 0.005 μg/mL. The EC50 of anti-human EGFR-MET bispecific antibody (Amivantamab) is approximately 0.36 μg/mL.

Inhibition of human EGF protein-induced reporter activity by anti-human EGF R neutralizing antibody.
This reporter cell was incubated with serial dilutions of antibodies in the presence of human EGF protein (Cat.No.EGF-H52H3) with a final concentration of 0.005 μg/mL. The EC50 of anti-human EGF R neutralizing antibody (Cetuximab) is approximately 0.005259 μg/mL.

Inhibition of human EGF protein-induced reporter activity by human EGF R small molecule inhibitor.
This reporter cell was incubated with serial dilutions of inhibitors in the presence of human EGF protein (Cat.No.EGF-H52H3) with a final concentration of 0.005 μg/mL. The EC50 of human EGF R small molecule inhibitor (Erlotinib) was approximately 0.2076 μM.

Response to human EGF protein (RLU).
The Human EGF R & (SRE-Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human EGF protein (Cat. No. EGF-H52H3). The EC50 was approximately 0.0034 μg/mL.

Response to human EGF protein (FOLD).
The Human EGF R & (SRE-Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human EGF protein (Cat. No. EGF-H52H3). The max induction fold was approximately 9.9.

Passage stability analysis by Signaling Bioassay.
The continuously growing Human EGF R & (SRE-Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human EGF protein. Human EGF protein stimulated response demonstrates passage stabilization (fold induction and EC50) across passage 5-23.