Human IL-5 R alpha/CD131 (Luc) HEK293 Reporter Cell

• Genetically modified cell lines best reflect MOA (Mechanism of Action)
• Higher activity and larger assay window for robust and reproducible cell-based bioassay
• Comprehensive application data to support assay development and validation
• Full tracible record, stringent quality control and validated cell passage stability
• Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
• Global commercial license assistance whenever regulatory filing is required

The Human IL-5 R alpha/CD131 (Luc) HEK293 Reporter Cell was engineered to not only express STAT5 signaling response element, but also express the receptors full length human IL-5 R alpha (Uniprot: Q01344-1) and CD131 (Uniprot: P32927-1). When stimulated with human IL-5 protein, the IL-5/ IL-5 R alpha interaction drives STAT5-mediated luminescence. Inhibition of IL-5 binding to IL-5 R alpha by either anti-IL-5 or anti-IL-5 R alpha antibodies results in a decrease in luminescence.

• Screen for anti-human IL-5 or anti-human IL-5 R alpha neutralizing antibody.

Adherent

NA

DMEM medium + 10% FBS

Serum-free cell cryopreservation medium

1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium

Frozen in liquid nitrogen.

Negative

Negative

See data sheet for detailed culturing and assay protocol.