TCF/LEF (Luc) HEK293 Reporter Cell

• Genetically modified cell lines best reflect MOA (Mechanism of Action)
• Higher activity and larger assay window for robust and reproducible cell-based bioassay
• Comprehensive application data to support assay development and validation
• Full tracible record, stringent quality control and validated cell passage stability
• Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
• Global commercial license assistance whenever regulatory filing is required

The TCF/LEF (Luc) HEK293 Reporter Cell was engineered to express TCF/LEF signaling response element driving luciferase expressing systems, designed for monitoring the activity of the Wnt/ß-catenin signaling pathway. When stimulated with human Wnt protein, receptor-mediated signaling can drive TCF/LEF-mediated luminescence. Inhibition of biological effect of human Wnt protein by corresponding inhibitors results in a decrease in luminescence.

• Monitor Wnt signaling pathway activity.

• Screen for Wnt/β-catenin targeted agents.

Adherent

Puromycin (2 μg/mL)

DMEM + 10% FBS

Serum-free cell cryopreservation medium

1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium

Frozen in liquid nitrogen.

Negative

Negative

See data sheet for detailed culturing and assay protocol.