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Assay Type | Sandwich-ELISA |
Analyte | Capsid protein p24 |
Format | 96T (8×12 strips) |
Reactivity | HIV (HV1-HXB2) |
Regulatory Status | RUO |
Sensitivity | <7.81 pg/mL |
Standard Curve Range | 250-7.81pg/mL |
Assay Time | 3 h 20 min |
Suitable Sample Type | For the quantitative determination of HIV-1 p24 in Cell Culture Supernatants, Plasma, Serum. |
Sample volume | 100 μL |
HIV-1 p24 ELISA Kit (Residue Testing) is based on the ELISA sandwich method and is used for the quantitative detection of Human Immunodeficiency Virus-1 p24 (HIV-1 p24) in samples from CAR-T product preparation processes where it is used as a lentivirus vector. It is designed to provide a reliable solution for CAR-T product quality assessment during drug development and CMC quality control stages.
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
ID | Components | Size |
RES050-C01 | Pre-coated Anti-HIV-1 p24 Antibody Microplate | 1 plate (8×12 strips) |
RES050-C02 | HIV-1 p24 Standard | 20 μg |
RES050-C03 | Biotin-Anti-HIV-1 p24 Antibody | 20 μg |
RES050-C04 | Streptavidin-HRP | 50 μL |
RES050-C05 | 10xWashing Buffer | 50 mL |
RES050-C06 | 2xDilution Buffer | 50 mL |
RES050-C07 | Substrate Solution | 12 mL |
RES050-C08 | Stop Solution | 7 mL |
RES050-C09 | 1xLysis Buffer | 12 mL |