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Assay Type | FRET |
Analyte | DNase I |
Format | 96T/480T |
Regulatory Status | RUO |
Sensitivity | 3.9×10⁻⁵ U |
Standard Curve Range | 0.0025 U-3.9×10⁻⁵ U |
Assay Time | 30 min |
Suitable Sample Type | For the quantitative determination of DNase I in the environment, some biological materials, common molecular biological reagents such as reaction buffers. |
Sample volume | 10 μL & 80 μL |
DNase Activity Assay Kit (Fluorescence) is based on the FRET method and is used to measure the presence of DNase in buffers, reagents, and other components within 30 minutes. It is designed to provide a rapid, highly sensitive, and quantitative universal detection tool for identifying DNase contamination that may affect experiments. Moreover, the DNase and RNase Activity Assay Kit (Fluorescence) has been designed to work seamlessly together for the simultaneous quantitative detection of DNases and RNases in a single sample.
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
1. Unopened kit should be stored at -25~-15℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 3 months from the date of opening.
Items | Size （96tests） | Size（480tests） |
DNase Substrate | 2 nmol | 10 nmol |
10X Reaction Buffer for DNase | 10 mL | 10 mL |
DNase I (1U/μL) | 20 μL | 50 μL |
TE Buffer (pH 8.0) | 1.5 mL | 6 mL |
Nuclease-free Water | 10 mL | 50 mL |
The DNase Activity Assay Kit (Fluorescence) is based on a fluorophore-labeled DNase substrate. When the sample does not contain DNase activity, the substrate is stable and does not produce a fluorescent signal; when the sample contains DNase activity, the substrate is degraded, resulting in a gradual enhanced fluorescence signal, the rate of increase in fluorescence signal is positively correlated with the number and activity of enzymes. Use a fluorescence microplate reader to measure at the wavelength of ex/em = 535/565 nm to determine whether the sample is contaminated by DNase.
