Human GM-CSF ELISA Kit

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製品番号/仕様
価格
数量
CRS-B007-96tests
$565.00
CRS-B007-480tests (96tests X 5)
$2545.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Product Overview

    Human GM-CSF ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human GM-CSF that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:

    1. Standards to calibrate with NIBSC/WHO standards for comparable results.
    2. Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
    3. High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
    4. Simplified and straightforward protocols and ready-to-use reagents to save assay time.

  • Product Specifications

    Assay Type
    Sandwich-ELISA
    Analyte
    GM-CSF
    Format
    96-wells plate breakable into 12 x 8 wells strips
    Reactivity
    Human
    Sensitivity
    10 pg/mL
    Assay Time
    1.75 hr
    Sample volume
    50 μL
    Range
    12.5 pg/mL-1600 pg/mL
    Sample Type
    Cell Culture Supernatants, Plasma, Serum.
    NIBSC Code
    88/646

    Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

  • Application

    The kit is developed for quantitative detection of natural and recombinant human GM-CSF in serum, plasma and cell culture supernatants.

    It is for research use only.

  • Workflow

    GM-CSF Workflow

  • Storage

    Unopened kit should be stored at 2℃-8℃ upon receiving. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Materials Provided

    ID
    Components
    Size
    CRB007-C01
    Pre-coated Anti-GM-CSF Antibody Microplate
    1 plate
    CRB007-C02
    Human GM-CSF Standard
    32 μg×2
    CRB007-C03
    Biotin-Anti-GM-CSF Antibody Con. Solution
    100 μL
    CRB007-C04
    Biotin-Antibody Dilution Buffer
    8 mL
    CRB007-C05
    Streptavidin-HRP Con. Solution
    500 μL
    CRB007-C06
    Streptavidin-HRP Dilution Buffer
    15 mL
    CRB007-C07
    20×Washing Buffer
    50 mL
    CRB007-C08
    1×Dilution Buffer
    15 mL×2
    CRB007-C09
    Substrate Solution
    12 mL
    CRB007-C10
    Stop Solution
    6 mL
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • Typical Data 

    Please refer to DS document for the assay protocol.

     GM-CSF TYPICAL DATA

    For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of GM-CSF is less than 10.0 pg/mL.

Validation
  • Intra-Assay Statistics

    Ten replicates of each of three samples containing different GM-CSF concentrations were tested in one assay. Acceptable criteria: CV<10%.

     GM-CSF INTRA-ASSAY STATISTICS
  • Inter-Assay Statistics

    Three samples containing different concentrations of GM-CSF were tested in independent assays. Acceptable criteria: CV<15%.

     GM-CSF INTER-ASSAY STATISTICS
  • Recovery

    Recombinant GM-CSF was spiked into five human serum samples, and then analyzed. The average recovery of GM-CSF for serum samples is 102.82%.

     GM-CSF RECOVERY

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