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Product Details
FAB
- Rapid conjugation: The entire operation process is just 3.5 hours.
- High Efficiency：Conjugation efficiency > 90%, without loss of antibodies.
- Simple operation: Without any purification steps.
Materials Provided
IDComponentsSizeALK-A005-C01Activated HRP1 vial/1 Reaction; 1 vial×3/3 ReactionsALK-A005-C02Crosslinking Reagent1 vial/1 Reaction; 1 vial×3/3 ReactionsALK-A005-C03Modifier Reagent1 vial/1 Reaction; 1 vial/3 ReactionsALK-A005-C04Quencher Reagent1 vial/1 Reaction; 1 vial/3 ReactionsProduct Overview
The HRP Antibody Labeling Kit is designed for the efficient and rapid conjugation of antibodies to horseradish peroxidase (HRP) via primary amine groups (such as lysine) on the antibody surface. The streamlined protocol allows for the preparation of conjugates in just 3.5 hours without purification steps or antibody loss. The resulting product is ready for direct use in standard ELISA experiments.
Note: The conjugates generated by this kit are not compatible with ELISA assays utilizing streptavidin-coated plates.
Storage
1. Unopened kit should be stored at -20℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
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Performance Data
Typical Data
Please refer to DS document for the assay protocol.
Binding ability of HRP labeled Anti-SN38 Antibody using Cat.No.ALK-A005:
Measured by its binding ability in a functional ELISA. Immobilized Human TROP-2, Fc Tag (Cat.No.TR2-H5253) at 1 μg/mL (100 μL/well) can bind Sacituzumab govitecan with a linear range of 0.01-1 ng/mL when detected by HRP labeled Anti-SN38 Antibody, Mouse IgG1 (0.5 μg/mL) (QC tested).
Binding ability of HRP labeled Monoclonal Anti-SN38 Antibody, Mouse IgG1 using Cat.No.ALK-A005 is superior to that of competitor Kit (Routinely tested).
Binding ability of HRP labeled Monoclonal Anti-AAV5 Antibody, Mouse Fc (10B4E6), Mouse IgG2a using Cat.No.ALK-A005 is superior to that of competitor Kit (Routinely tested).
Binding ability of HRP labeled Monoclonal Anti-Rabies virus (strain CVS-11) Nucleoprotein Antibody, Mouse IgG2b (2A12) using Cat.No.ALK-A005 is superior to that of competitor Kit (Routinely tested).
Binding ability of HRP labeled Monoclonal Anti-Camelid VHH Antibody, Rabbit IgG (M1A11), Rabbit IgG using Cat.No.ALK-A005 is superior to that of competitor Kit (Routinely tested).
Binding ability of HRP labeled Monoclonal Anti-Human CTLA-4 Antibody, Human IgG1 using Cat.No.ALK-A005 is superior to that of competitor Kit (Routinely tested).
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