pH-Sensitive Fluorescent Dye Antibody Labeling Kit (Em660, 100μg/Reaction)

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Cat. No. / Size
Price
Qty
ALK-A004-3Reactions
$700.00
ETA of in-stock products:2 business days

Product Details

  • FAB

    1. Direct labeling - Eliminates secondary antibodies for a more direct signal, smaller molecular weight, and greater analytical flexibility.
    2. High signal to noise ratio - Strong fluorescence with minimal background.
    3. pH sensitive - Bright emission in acidic milieu.
    4. Rapid fluorescent labeling - Only 30 minutes of hands-on time.
    5. Broad antibody compatibility​ - Labeling via antibody amines works across different antibody species and subtypes.
  • Materials Provided

    BoxA(-20°C)
    ID
    Components
    Size
    ALK-A004-C01
    Activated pH-Sensitive Fluorescent Dye (Em660)
    1 vialx3
    ALK-A004-C02
    1M NaHCO3
    1 vial
    ALK-A004-C03
    Quencher Reagent
    1 vial
    BoxB(4°C)
    ID
    Components
    Size
    ALK-A004-1-C01
    1xPBS (pH7.2-7.4), 11% Trehalose
    5 mL
    ALK-A004-1-C02
    Desalting Spin column 0.5 mL
    3 per
  • Product Overview

    The pH-sensitive dye (Em660) cannot only significantly enhance the fluorescence signal in an acidic environment but also minimize background noise effectively, which has an excitation wavelength of 643 nm and an emission wavelength of 660 nm.

    pH-Sensitive Fluorescent Dye Antibody Labeling Kit provides a rapid and simple method to covalently label antibodies with pH-sensitive fluorescent dye by targeting primary amine groups.

  • Application

    pH-sensitive Dye Antibody Labeling Kit (Em660) provides a rapid and simple method for achieving covalent coupling between pH-sensitive fluorescent dye and antibody molecules. The conjugation reaction requires simple purification via spin column chromatography with straightforward operation, and the prepared dye-labeled antibody can be used for the detection of antibody internalization efficiency in cells.

    It is for research use only.

  • Storage

    The transport temperature for the kit is 2-8°C.

    • BoxA: Unopened kit should be stored at -20℃ upon receiving.

    • BoxB: Unopened kit should be stored at 2-8℃ upon receiving.

    Find the expiration date on the outside packaging and do not use reagents past their expiration date.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-FACS 

    Please refer to DS document for the assay protocol.

     pH-sensitive fluorescent dye FACS

    Anti-Her2 Ab and Human IgG1 isotype control were labeled using pH-Sensitive Fluorescent Dye Antibody Labeling Kit (Cat. No. ALK-A004). SK-BR-3 cells were treated with Dye Labeled Anti-Her2 Ab and Dye Labeled Isotype Control separately at 37℃ for 4 hours, then analysis by Flow cytometric. APC signal was used to evaluate the internalization signal.

  •  pH-sensitive fluorescent dye FACS

    Anti-CD20 Ab and Human IgG1 isotype control were labeled using pH-Sensitive Fluorescent Dye Antibody Labeling Kit (Cat. No. ALK-A004). Raji cells were treated with Dye Labeled Anti-CD20 Ab and Dye Labeled Isotype Control separately at 37℃ for 4 hours, then analysis by Flow cytometric. APC signal was used to evaluate the internalization signal.

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