SARS-CoV-2 Spike S1 (B.1.1.529) Specific ELISA Kit (For Vaccine Development)

The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has posed a serious threat to human health. A rapid and effective assay kit detecting the levels of SARS-CoV-2 Spike S1 is urgently needed to accelerate the development of COVID-19 vaccines.

The kit is developed for specific detection of SARS-CoV-2 Spike S1 (B.1.1.529) in vaccine samples, which can meet the needs of vaccine developers to establish antigen quantification methods for preclinical evaluation, vaccine production and quality control,and realize accurate quantification of vaccine antigen contents for COVID-19 vaccines of Omicron-specific boosters.

It is for research use only.

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

1. Store the unopened kit at 2-8°C upon receipt.

2. Locate the expiration date on the outer packaging and do not use reagents beyond their expiration date.

3. The opened kit should be stored according to the storage conditions listed in the Components Table. The shelf life of the opened kit is 30 days from the date of opening.

This assay kit employs a standard sandwich-ELISA format,providing a rapid detection of SARS-CoV-2 Spike S1.The kit consists of microplate pre-coated with Anti-SARS-CoV-2 Spike S1 Antibody,SARS-CoV-2 Spike S1 as Control,HPR-Anti-SARS-CoV-2 Spike S1 Antibody and buffers.

Your experiment will include 5 simple steps:

a) Bring all reagents and samples to room temperature (20℃-25℃) before use.

b) Add the sample and Control diluted by Dilution Buffer to the plate.

c) Add the HRP-conjugated antibody diluted by Dilution Buffer to the plate.

d) Wash the plate and add TMB.

e) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.