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Assay Type | Sandwich-ELISA |
Analyte | T7 RNA polymerase |
Format | 96T (8×12 strips) |
Reactivity | Human |
Regulatory Status | RUO |
Sensitivity | <0.78 ng/mL |
Standard Curve Range | 0.78 ng/mL-50 ng/mL |
Assay Time | 3 h 20 min |
Suitable Sample Type | For the quantitative determination of T7 RNA polymerase in Cell Culture Supernatants. |
Sample volume | 100 μL |
T7 RNA Polymerase ELISA Kit (Residue Testing) is based on the ELISA sandwich method and is designed to detect and quantitatively determine T7 RNA Polymerase residues in mRNA drugs. The kit contains T7 RNA Polymerase (ACROBiosystems, cat# T7E-E5143) to ensure accurate assay results and is designed to provide a reliable solution for monitoring and controlling T7 RNA Polymerase residues during the production of mRNA products. It can also be used as a universal detection tool for the quantitative determination of T7 RNA Polymerase.
The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
ID | Components | Size |
RES018-C01 | Pre-coated Anti-T7 RNA polymerase Antibody Microplate | 1 plate (8×12 strips) |
RES018-C02 | T7 RNA polymerase Standard | 100 μL |
RES018-C03 | Biotin-Anti-T7 RNA polymerase Antibody | 150 μL |
RES018-C04 | Streptavidin-HRP | 50 μL |
RES018-C05 | 20xWashing Buffer | 50 mL |
RES018-C06 | Biotin-Antibody and Streptavidin-HRP Dilution Buffer | 50 mL |
RES018-C07 | Standard and Sample Dilution Buffer (5x) | 30 mL |
RES018-C08 | Substrate Solution | 12 mL |
RES018-C09 | Stop Solution | 7 mL |