resDetect™ TrypLE ELISA Kit

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RES-A057-96tests
$280.00
In Stock - Delivery in 1-2 business days
RES-A057
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Product Specifications

Assay Type
Sandwich-ELISA
Analyte
TrypLE
Format
96T (8×12 strips)
Regulatory Status
RUO
Sensitivity
<12.5 pg/mL
Standard Curve Range
12.5 pg/mL-400 pg/mL
Assay Time
2 h
Suitable Sample Type
For the detection and quantitative determination of TrypLE in cell culture sample.
Sample volume
100 μL

Product Overview

TrypLE (Trypsin Like Enzyme) is an animal origin-free, recombinant enzyme. It's an important raw material from the acquisition of early primary cells to the subsequent passage and digestion of adherent cells, and then to large-scale cell factory or microcarrier reactor culture.The amount of trypLE should be strictly controlled when digesting cells, and the detection of trypLE residues is also an important link to ensure the normal growth of cells.Because a large amount of trypLE was used for digestion in the culture stage, it is necessary to test for trypLE of the downstream part of the production.

Application

The kit is developed for the detection and quantitative determination of residual trypLE in bio-manufacturing down-stream processing fast and reliable. The assay is perfectly suited for process development, in-process control and final quality control.

It is for research use only.

Storage

1. Store the unopened kit at 2-8°C upon receipt.

2. Locate the expiration date on the outer packaging and do not use reagents beyond their expiration date.

Materials Provided

ID
Components
Size
RES057-C01
Pre-coated Anti-TrypLE Antibody Microplate
1 plate (8×12 strips)
RES057-C02
TrypLE Standard
15 μg
RES057-C03
HRP-Anti-TrypLE Antibody
15 μg
RES057-C04
10×Washing Buffer
50 mL
RES057-C05
2×Dilution Buffer
50 mL
RES057-C06
Substrate Solution
12 mL
RES057-C07
Stop Solution
7 mL