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Assay Type | Sandwich-ELISA |
Analyte | TrypLE |
Format | 96T (8×12 strips) |
Regulatory Status | RUO |
Sensitivity | <12.5 pg/mL |
Standard Curve Range | 12.5 pg/mL-400 pg/mL |
Assay Time | 2 h |
Suitable Sample Type | For the detection and quantitative determination of TrypLE in cell culture sample. |
Sample volume | 100 μL |
TrypLE (Trypsin Like Enzyme) is an animal origin-free, recombinant enzyme. It's an important raw material from the acquisition of early primary cells to the subsequent passage and digestion of adherent cells, and then to large-scale cell factory or microcarrier reactor culture.The amount of trypLE should be strictly controlled when digesting cells, and the detection of trypLE residues is also an important link to ensure the normal growth of cells.Because a large amount of trypLE was used for digestion in the culture stage, it is necessary to test for trypLE of the downstream part of the production.
The kit is developed for the detection and quantitative determination of residual trypLE in bio-manufacturing down-stream processing fast and reliable. The assay is perfectly suited for process development, in-process control and final quality control.
It is for research use only.
1. Store the unopened kit at 2-8°C upon receipt.
2. Locate the expiration date on the outer packaging and do not use reagents beyond their expiration date.
ID | Components | Size |
RES057-C01 | Pre-coated Anti-TrypLE Antibody Microplate | 1 plate (8×12 strips) |
RES057-C02 | TrypLE Standard | 15 μg |
RES057-C03 | HRP-Anti-TrypLE Antibody | 15 μg |
RES057-C04 | 10×Washing Buffer | 50 mL |
RES057-C05 | 2×Dilution Buffer | 50 mL |
RES057-C06 | Substrate Solution | 12 mL |
RES057-C07 | Stop Solution | 7 mL |