PE-Labeled Human ACE2 / ACEH Protein, His TagStar Staining

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AC2-HP2H3-25ug
$360.00
In Stock - Delivery in 2-3 business days
AC2-HP2H3-200ug
$2245.00
In Stock - Delivery in 2-3 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    ACE-2, ACEH, ACE2

  • Source

    PE-Labeled Human ACE2, His Tag (AC2-HP2H3) is expressed from human 293 cells (HEK293). It contains AA Gln 18 - Ser 740 (Accession # Q9BYF1-1).

    Predicted N-terminus: Gln 18

    Request for sequence
  • Molecular Characterization

    ACE2 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

  • Conjugate

    PE

    Excitation Wavelength: 488 nm / 561 nm

    Emission Wavelength: 575 nm

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • Bioactivity-ELISA

     ACE2 ELISA

    Immobilized SARS S protein RBD, His Tag (Cat. No. SPD-S52H6) at 5 μg/mL (100 μL/well) can bind PE-Labeled Human ACE2, His Tag (Cat. No. AC2-HP2H3) with a linear range of 0.078-20 μg/mL (QC tested).

    Protocol
  • Bioactivity-Flow Cytometry

     ACE2 FACS

    Biotinylated SARS-CoV-2 Spike RBD Protein, His, Avitag™ (MALS verified) (Cat. No. SPD-C82E9) at 10pg/bead on Recombinant Streptavidin Protein (Cat. No. STN-N5116) at 2.5pg/bead were conjugated to fluorochrome-encoded microspheres. Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG1 (AM359b) (MALS verified) (Cat. No. SPD-M265) competes with PE-Labeled Human ACE2, His tag (Cat. No. AC2-HP2H3) for binding to Biotinylated SARS-CoV-2 Spike RBD Protein encapsulated beads with a linear range of 16.25-8320 ng/mL.

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よくある質問

  • product

    Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?

    In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.


    Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.


    To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.


    To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.


    Supporting Document


バックグランド

Angiotensin-converting enzyme 2 (ACE2) is also known as ACEH (ACE homolog), is an integral membrane protein with considerable homologous to ACE, which belongs to the peptidase M2 family. ACE2 is an exopeptidase that catalyses the conversion of angiotensin I to the nonapeptide angiotensin, or the conversion of angiotensin II to angiotensin 1-7. ACE2 may be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, ACE-2 serve as functional receptor for the spike glycoprotein of both coronaviruses. ACE2 is activated by chloride and fluoride, but not bromide and Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat. ACE2 is active from pH 6 to 9, and the optimum pH is 6.5 in the presence of 1 M NaCl.

最新の進展

 
医薬品開発の進捗
  • 英語名:

    Angiotensin-converting enzyme 2

  • カテゴリ:

  • 市販の医薬品数:

    0 詳細

  • 治験医薬品数:

    7 詳細

  • 最新の開発ステージ:

    Phase 2 Clinical

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