Mink ACE2 / ACEH Protein, His Tag (MALS & SPR verified)

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製品番号/仕様
価格
数量
AC2-M52H3-50ug
$435.00
AC2-M52H3-1mg (500ug X 2)
$3170.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    ACE-2, ACEH, ACE2

  • Source

    Mink ACE2, His Tag (AC2-M52H3) is expressed from human 293 cells (HEK293). It contains AA Gln 18 - Thr 740 (Accession # QPL12211.1).

    Predicted N-terminus: Gln 18

    Request for sequence
  • Molecular Characterization

    ACE2 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 86.3 kDa. The protein migrates as 95-105 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM Nacl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    ACE2 SDS-PAGE

    Mink ACE2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • SEC-MALS

    ACE2 SEC-MALS

    The purity of Mink ACE2, His Tag (Cat. No. AC2-M52H3) is more than 85% and the molecular weight of this protein is around 175-195 kDa verified by SEC-MALS.

    Report
  • Bioactivity-SPR

     ACE2 SPR

    Mink ACE2, His Tag (Cat. No. AC2-M52H3) immobilized on CM5 Chip can bind SARS-CoV-2 S protein RBD (Y453F), His Tag with an affinity constant of 0.178 μM as determined in a SPR assay (Biacore T200) (QC tested).

    Protocol
  •  ACE2 SPR

    Mink ACE2, His Tag (Cat. No. AC2-M52H3) immobilized on CM5 Chip can bind SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H3) with an affinity constant of 8.34 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

    Protocol

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バックグランド

Angiotensin-converting enzyme 2 (ACE2) is also known as ACEH (ACE homolog), is an integral membrane protein with considerable homologous to ACE, which belongs to the peptidase M2 family. ACE2 is an exopeptidase that catalyses the conversion of angiotensin I to the nonapeptide angiotensin, or the conversion of angiotensin II to angiotensin 1-7. ACE2 may be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, ACE-2 serve as functional receptor for the spike glycoprotein of both coronaviruses. ACE2 is activated by chloride and fluoride, but not bromide and Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat. ACE2 is active from pH 6 to 9, and the optimum pH is 6.5 in the presence of 1 M NaCl.

最新の進展

 
医薬品開発の進捗
  • 英語名:

    Angiotensin-converting enzyme 2

  • カテゴリ:

  • 市販の医薬品数:

    0 詳細

  • 治験医薬品数:

    7 詳細

  • 最新の開発ステージ:

    Phase 2 Clinical

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