Alkaline phosphatase (4U/ul)

Product Details

Alkaline Phosphatases (APs) is derived from Antarctic bacterium and expressed in E. coli. APs nonspecifically catalyzes the dephosphorylation of 5´ and 3´ ends of DNA and RNA phosphomonoesters. Also, APs Hydrolyses ribo-, as well as deoxyribonucleoside triphosphates (NTPs and dNTPs).

Application

Dephosphorylation 5´ and 3´ ends of DNA and RNA.
Dephosphorylation of cloning vector DNA to prevent recircularization during ligation.
Dephosphorylation of DNA prior to end-labeling using T4 Polynucleotide Kinase.
Treatment of dNTPs in PCR reactions prior to sequencing or SNP analysis.

Unit Definition

One unit is defined as the amount of enzyme that will dephosphorylate 1 µg of pUC19 vector DNA cut with a restriction enzyme generating 5´ recessed ends in 30 minutes at 37°C.

Purity

>90% as determined by SDS-PAGE.

Notes

Alkaline Phosphatase is inhibited by metal chelators (e.g. EDTA), inorganic phosphate and phosphate analogs.

Alkaline Phosphatase activity is decreased in the presence of reducing agents (DTT, β-ME).

Formulation

Supplied as 0.2 μm filtered solution in 10 mM Tris, pH7.4 with glycerol as protectant.

Contact us for customized product form or formulation.

Shipping and Storage

This product is supplied and shipped on dry ice.

This product is stable after storage at:

1. The product MUST be stored at -20°C or lower upon receipt.
2. -20°C for 12 months under sterile conditions.