Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?

In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions. Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage. To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance. To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt. Supporting Document Validation Report for Accelerated Stability Study of 11 Lyophilized Proteins

Human APRIL / TNFSF13 Protein, His,Flag Tag, active trimer, low endotoxin (Mass Spec & MALS verified)

製品詳細

Synonyms
APRIL, TNFSF13, TALL-2, TRDL-1, CD256, TALL2, ZTNF2
Source
Human APRIL Protein, His,Flag Tag (APL-H52D1) is expressed from human 293 cells (HEK293). It contains AA Gln 111 - Leu 250 (Accession # O75888-1) trimer Design.
Predicted N-terminus: His
Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus, followed by a flag tag.
The protein has a calculated MW of 50.2 kDa. The protein migrates as 55-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
Mass Spectrometry
1. Sequence Coverage
  Human APRIL Protein, His,Flag Tag(APL-H52D1) was digested with trypsin and characterized by LC-MS/MS peptide mapping, achieving 100% sequence coverage (Routinely tested).
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System

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データ表示

SDS-PAGE
Human APRIL Protein, His,Flag Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human APRIL Protein, His,Flag Tag (Cat. No. APL-H52D1) is more than 95% and the molecular weight of this protein is around 50-75 kDa verified by SEC-MALS.
Report

Bioactivity-ELISA
Immobilized Human BCMA, Fc Tag (Cat. No. BC7-H5254) at 2 μg/mL (100 μL/well) can bind Human APRIL Protein, His,Flag Tag (Cat. No. APL-H52D1) with a linear range of 10-39 ng/mL (QC tested).
Protocol
Immobilized Human APRIL Protein, His,Flag Tag (Cat. No. APL-H52D1) at 1 μg/mL (100 μL/well) can bind Human TACI Protein, Fc Tag with a linear range of 0.8-25 ng/mL (Routinely tested).
Protocol
Immobilized Human APRIL Protein, His,Flag Tag (Cat. No. APL-H52D1) at 1 μg/mL (100 μL/well) can bind Human Anti-APRIL (Sibeprenlimab), Human IgG2 (S257A) | Human Kappa with a linear range of 0.06-4 ng/mL (Routinely tested).
Protocol

Bioactivity-BLI
Loaded Human APRIL Protein, His,Flag Tag (Cat. No. APL-H52D1) on HIS1K Biosensor, can bind Human BCMA, Fc Tag (Cat. No. BC7-H5254) with an affinity constant of 1.88 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human APRIL Protein, His,Flag Tag (Cat. No. APL-H52D1) on HIS1K Biosensor, can bind Human TACI Protein, Fc Tag with an affinity constant of 75 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol

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product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.

Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.

To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.

To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.

Supporting Document
- Validation Report for Accelerated Stability Study of 11 Lyophilized Proteins

バックグランド

APRIL(a proliferation-inducing ligand) is also known as Tumor necrosis factor ligand superfamily member 13, TALL-2, TRDL-1, CD256, TNFSF13, cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. APRIL is a cytokine of the tumor necrosis factor family associated mainly with hematologic malignancies. The closely related TNF family ligands B cell activation factor (BAFF) and a proliferation-inducing ligand (APRIL) serve in the generation and maintenance of mature B-lymphocytes. Both BAFF and APRIL assemble as homotrimers that bind and activate several receptors that they partially share. BAFF-APRIL heteromers of different stoichiometries have distinct receptor-binding properties and activities. In addition, expression of APRIL was regulated by miR-145 in GC cells.

人気検索語:

Human APRIL / TNFSF13 Protein, His,Flag Tag, active trimer, low endotoxin (Mass Spec & MALS verified)AIDD‑Ready

製品詳細

Synonyms

Source

Molecular Characterization

Mass Spectrometry

Sequence Coverage

Endotoxin

Purity

Formulation

Reconstitution

Shipping and Storage

ACRO Quality Management System

お客様はこちらも閲覧

データ表示

SDS-PAGE

SEC-MALS

Bioactivity-ELISA

Bioactivity-BLI

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