製品詳細
Synonyms
NGF, NGFB, Beta-NGF, HSAN5, MGC161426, MGC161428
Source
Mouse Beta-NGF Protein, Tag Free (BEF-M5213) is expressed from human 293 cells (HEK293). It contains AA Ser 122 - Gly 241 (Accession # P01139).
Predicted N-terminus: Ser 122
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries no "tag".
The protein has a calculated MW of 13.5 kDa. The protein migrates as 12-14 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Sterility
Negative
Mycoplasma
Negative
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 20 mM NaAC, 150 mM NaCl, pH5.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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データ表示
SDS-PAGE

Mouse Beta-NGF Protein, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS

The purity of Mouse Beta-NGF Protein, Tag Free (Cat. No. BEF-M5213) is more than 95% and the molecular weight of this protein is around 32-45 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA

Immobilized Mouse Beta-NGF Protein, Tag Free (Cat. No. BEF-M5213) at 1 μg/mL (100 μL/well) can bind Human TrkA (33-417), Fc Tag (Cat. No. TRA-H5254) with a linear range of 0.2-16 ng/mL (QC tested).
Protocol
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- 英語名:
Nerve growth factor
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