- 製品詳細
- データ表示
- Reviews & Citations
- Resources


CTSB, CPSB, APPS
Human Cathepsin B, His Tag (CTB-H5222) is expressed from human 293 cells (HEK293). It contains AA Arg 18 - Ile 339 (Accession # P07858-1 (L26V)).

This protein carries a polyhistidine tag at the C-terminus. The Human Cathepsin B will be further processed into mature form (Phe 74 - Ile 339). The protein has a calculated MW of 36.7 kDa (pro-form) and 29.5 kDa (mature-form). The protein migrates as 30-32 kDa and 38-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Human Cathepsin B, His Tag(CTB-H5222) was digested with trypsin and characterized by LC-MS/MS peptide mapping, achieving chain1: 98.8% and chain2: 98.5% sequence coverage (Routinely tested).
chain1: Arg-Ser-Arg-Pro-Ser-Phe-His-Pro-Val-Ser-Asp-Glu-Leu-Val-Asn
chain2: Phe-Thr-Glu-Asp-Leu-Lys-Leu-Pro-Ala-Ser-Phe-Asp-Ala-Arg-Glu
(Routinely tested)
Less than 1.0 EU per μg by the LAL method / rFC method.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH8.0 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.