Human CD27 Ligand / CD70 Protein, His,Flag Tag, active trimer

AIDD‑Ready
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CDL-H52DaAI-250ug
$1,040.00
CDL-H52DaAI-500ug (250ug X 2)
$1,660.00
CDL-H52DaAI
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Application

Human CD27 Ligand Protein, His,Flag Tag AIDD-Ready protein is designed for direct use in SPR assays. It is supplied in a sugar-free, glycerol-free formulation to eliminate refractive index interference in SPR. No desalting or buffer exchange is required before use, simplifying the SPR workflow and supporting high-throughput experimental validation to accelerate the transition from AI prediction to wet-lab validation.

Features and Advantages
  • Directly compatible with SPR: No desalting or buffer exchange required; supplied as a liquid to reduce sample preparation and improve experimental efficiency.
  • High batch-to-batch consistency: Each lot is released using SPR QC to ensure experimental reproducibility.
  • Validated with representative drugs: SPR binding activity is validated using representative drug antibodies to ensure suitability for drug screening and evaluation.
Synonyms

CD70, CD27LG, TNFSF7, TNFSF7G, CD27L

Source

Human CD27 Ligand Protein, His,Flag Tag (CDL-H52DaAI) is expressed from human 293 cells (HEK293). It contains AA Ser 52 - Pro 193 (Accession # P32970-1).

Predicted N-terminus: His

Molecular Characterization

This protein carries a polyhistidine tag at the N-terminus, followed by a flag tag.

The protein has a calculated MW of 49.3 kDa. The protein migrates as 53-58 kDa, 95-105 kDa, 130-140 kDa and >180 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Purity

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Formulation

Formulated in a proprietary trehalose-free buffer, supplied ready-to-use for SPR/BLI analysis - no buffer exchange required.

For detailed formulation, please refer to the batch-specific COA.

Shipping and Storage

This product is supplied and shipped on dry ice.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • The product MUST be stored at -70°C or lower upon receipt;
  • -70°C for 12 months under sterile conditions.

Background

Cluster of Differentiation 70 (CD70) is also known as CD27 ligand (CD27L / CD27LG), TNFSF7, TNFSF7G, is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and is a surface antigen found on activated T-and B-lymphocytes and mature dendritic cells. Binding of CD70 to its receptor CD27 induces in priming, effector functions, differentiation and memory formation of T-cells, and thus is invloved in the biological processes including T-cell activation, the proliferation of costimulated T-cells, as well as the generation of cytolytic T-cells. CD70 on T cells provides costimulatory signals that are required for T cell proliferation, clonal expansion and the promotion of effector T cell formation. CD70 on mouse B cell has been shown to inhibit terminal differentiation of activated B cells into plasma cells and enhances commitment to memory B cell responses. CD70 induces proliferation and IFNγ production, on NK cells.

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