APC-Labeled Human CD27 Ligand / CD70 Protein, His TagStar Staining

製品番号/仕様
価格
数量
CDL-HA248-25tests
$360.00
CDL-HA248-200tests
$2245.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    CD70, CD27LG, TNFSF7, TNFSF7G, CD27L

  • Source

    APC-Labeled Human CD27 Ligand Protein, His Tag (CDL-HA248) is produced via conjugation of APC to Human CD27 Ligand Protein, His Tag with a new generation site-specific technology under Star Staining labeling platform. Human CD27 Ligand Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Ser 52 - Pro 193 (Accession # P32970-1).

    Predicted N-terminus: His

  • Molecular Characterization

    This protein carries a polyhistidine tag at the N-terminus.

  • Conjugate

    APC

    Excitation Wavelength: 640 nm

    Emission Wavelength: 661 nm

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 0.2% BSA, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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  • Evaluation of CAR expression

    FACS Analysis of Anti-CD27 Ligand CAR Expression

     CD27 Ligand CAR_T

    5e5 of anti-CD27 Ligand CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of APC-Labeled Human CD27 Ligand Protein, His Tag (Cat. No. CDL-HA248) and negative control protein respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). APC signal was used to evaluate the binding activity (QC tested).

    Protocol

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バックグランド

Cluster of Differentiation 70 (CD70) is also known as CD27 ligand (CD27L / CD27LG), TNFSF7, TNFSF7G, is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and is a surface antigen found on activated T-and B-lymphocytes and mature dendritic cells. Binding of CD70 to its receptor CD27 induces in priming, effector functions, differentiation and memory formation of T-cells, and thus is invloved in the biological processes including T-cell activation, the proliferation of costimulated T-cells, as well as the generation of cytolytic T-cells. CD70 on T cells provides costimulatory signals that are required for T cell proliferation, clonal expansion and the promotion of effector T cell formation. CD70 on mouse B cell has been shown to inhibit terminal differentiation of activated B cells into plasma cells and enhances commitment to memory B cell responses. CD70 induces proliferation and IFNγ production, on NK cells.

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医薬品開発の進捗
  • 英語名:

    CD70 antigen

  • カテゴリ:

  • 市販の医薬品数:

    0 詳細

  • 治験医薬品数:

    37 詳細

  • 最新の開発ステージ:

    Phase 2 Clinical

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