APC-Labeled Human CD3 epsilon&CD3 gamma Heterodimer Protein, Fc,His Tag&Fc,Flag Tag

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CDG-HA2W3-25ug
$450.00
In Stock - Delivery in 2-3 business days
CDG-HA2W3-200ug
$2803.00
In Stock - Delivery in 2 weeks
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    CD3 epsilon & CD3 gamma, CD3E & CD3G

  • Source

    APC-Labeled Human CD3E & CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (CDG-HA2W3) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 & Gln 23 - Ser 116 (Accession # P07766-1 & P09693-1). It is the APC labeled form of Human CD3E&CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDG-H52W9).

    Predicted N-terminus: Asp 23 & Gln 23

    Request for sequence
  • Molecular Characterization

    CD3E & CD3G Structure

    Other Tags and Version Biotin & Other Labeled Version

    APC-Labeled Human CD3E & CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 39.2 kDa (CD3E) and 38.6 kDa (CD3G). The subunit CD3E carries a human IgG1 Fc fragment at the C-terminus, followed by a polyhistidine tag. The subunit CD3G carries a human IgG1 Fc fragment at the C-terminus, followed by a flag tag.

  • Conjugate

    APC

    Excitation Wavelength: 640 nm

    Emission Wavelength: 661 nm

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • Bioactivity-ELISA

     CD3E & CD3G ELISA

    Immobilized APC-Labeled Human CD3E & CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDG-HA2W3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG1 (UCHT1) (Cat. No. CDE-M532) with a linear range of 0.1-4 ng/mL (QC tested).

    Protocol

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よくある質問

  • product

    Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?

    In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.


    Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.


    To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.


    To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.


    Supporting Document


バックグランド

T-cell surface glycoprotein CD3 delta & CD3 gamma chain, also known as CD3D & CD3G or CD3D&CD3G respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

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