Human CD4 / LEU3 Protein, His Tag (MALS verified)DMF

製品番号/仕様
価格
数量
LE3-H5228-200ug
$360.00
LE3-H5228-1mg (200ug X 5)
$1545.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    CD4, CD4mut, LEU3

  • Source

    Human CD4, His Tag (LE3-H5228) is expressed from human 293 cells (HEK293). It contains AA Lys 26 - Trp 390 (Accession # P01730).

    Predicted N-terminus: Lys 26

    Request for sequence
  • Molecular Characterization

    CD4 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 41.5 kDa. The protein migrates as 47-54 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.1 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 12 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    CD4 SDS-PAGE

    Human CD4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    CD4 SEC-MALS

    The purity of Human CD4, His Tag (Cat. No. LE3-H5228) is more than 95% and the molecular weight of this protein is around 40-50 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     CD4 ELISA

    Immobilized Ibalizumab at 1 μg/mL (100 μL/well) can bind Human CD4, His Tag (Cat. No. LE3-H5228) with a linear range of 0.8-6 ng/mL (QC tested).

    Protocol
  •  CD4 ELISA

    Immobilized Human CD4, His Tag (Cat. No. LE3-H5228) at 1 μg/mL (100 μL/well) can bind Ibalizumab with a linear range of 0.2-13 ng/mL (Routinely tested).

    Protocol
  •  CD4 ELISA

    Immobilized Human CD4, His Tag (Cat. No. LE3-H5228) at 2 μg/mL (100 μL/well) can bind Anti-CD4 with a linear range of 0.5-16 ng/mL (Routinely tested).

    Protocol

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バックグランド

T-cell surface glycoprotein CD4 is also known as T-cell surface antigen T4/Leu-3. CD4 contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CD4 is accessory protein for MHC class-II antigen/T-cell receptor interaction. CD4 induces the aggregation of lipid rafts. CD4 is a primary receptor used by HIV-1 to gain entry into host T cells. HIV infection leads to a progressive reduction of the number of T cells possessing CD4 receptors. Therefore, medical professionals refer to the CD4 count to decide when to begin treatment for HIV-infected patients.

最新の進展

 
医薬品開発の進捗
  • 英語名:

    T-cell surface antigen CD4

  • カテゴリ:

  • 市販の医薬品数:

    1 詳細

  • 治験医薬品数:

    25 詳細

  • 最新の開発ステージ:

    Approved

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