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製品詳細
Synonyms
CD8A, CD8, Leu2, MAL, p32
Source
Alexa Fluor 488-Labeled Human CD8 alpha Protein, His Tag (CDA-HA2H6) is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Asp 182 (Accession # P01732-1). It is the Alexa Fluor™ 488 labeled form of Human CD8 alpha, His Tag (Cat. No. CDA-H52H3).
Predicted N-terminus: Ser 22
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 19.5 kDa. The protein migrates as 23-30 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Conjugate
AF488
Excitation Wavelength: 488 nm
Emission Wavelength: 517 nm
Labeling
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with AF488 using standard chemical labeling method. The residual AF488 is removed by molecular sieve treatment during purification process.
Protein Ratio
The AF488 to protein molar ratio is 1-3.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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データ表示
SDS-PAGE
Alexa Fluor 488-Labeled Human CD8 alpha Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Nanoflow Cytometry (tLNP)
2-3*10¹⁰ particles/mL of tLNP-hCD8-Ab (tLNP Coupling CD8 Ab) were stained with 1 μg/mL of Alexa Fluor™ 488-Labeled Human CD8 alpha Protein, His Tag (Cat. No. CDA-HA2H6) , AF488 signal was used to evaluate the binding activity (Routinely tested).
Protocol
Bioactivity-ELISA
Immobilized Alexa Fluor 488-Labeled Human CD8 alpha Protein, His Tag (Cat. No. CDA-HA2H6) at 5 μg/mL (100 μL/well) can bind Anti-CD8 alpha Antibody, Human IgG1 with a linear range of 0.2-16 ng/mL (Routinely tested).
Protocol
Immobilized Anti-CD8 alpha Antibody, Human IgG1 at 5 μg/mL (100 μL/well) can bind Alexa Fluor 488-Labeled Human CD8 alpha Protein, His Tag (Cat. No. CDA-HA2H6) with a linear range of 1-16 ng/mL (Routinely tested).
Protocol
Bioactivity-Flow Cytometry
1e5 of Mouse Anti-CD8 antibody coupled beads (5.5 μm) were stained with different concentration of Alexa Fluor 488-Labeled Human CD8 alpha Protein, His Tag (Cat. No. CDA-HA2H6) and negative control protein respectively, AF488 signal was used to evaluate the binding activity (QC tested).
Protocol
1e5 of Mouse Anti-CD8 antibody coupled beads (5.5 μm) were stained with different concentration of Alexa Fluor 488-Labeled Human CD8 alpha Protein, His Tag (Cat. No. CDA-HA2H6) and negative control protein respectively, AF488 signal was used to evaluate the binding activity (QC tested).
Protocol
Bioactivity-Stability
Binding activity of three different lots of Alexa Fluor 488-Labeled Human CD8 alpha Protein, His Tag against Mouse Anti-CD8 antibody coupled beads (5.5 μm) was evaluated by flow cytometry. The result shows very high batch-to-batch consistency.
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よくある質問
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
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最新の進展
医薬品開発の進捗- 英語名：
Cluster of differentiation 8
- カテゴリ：
- 市販の医薬品数：
0 詳細
- 治験医薬品数：
18 詳細
- 最新の開発ステージ：
Phase 3 Clinical
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