Alexa Fluor™ 488 & Biotinylated-Labeled Mouse CD8 alpha Protein, His,Avitag™

Synonyms

CD8A, CD8, Leu2, MAL, p32

Source

Alexa Fluor 488 & Biotinylated-Labeled Mouse CD8 alpha Protein, His,Avitag (CDA-MA2E3) is expressed from human 293 cells (HEK293). It contains AA Lys 28 - Tyr 196 (Accession # P01731-1). It is the Alexa Fluor™ 488 labeled form of Biotinylated Mouse CD8 alpha, His,Avitag (Cat. No. CDA-M82E8).

Predicted N-terminus: Lys 28

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 22.5 kDa. The protein migrates as 33-40 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Conjugate

AF488

Excitation Wavelength: 488 nm

Emission Wavelength: 517 nm

Labeling

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with AF488 using standard chemical labeling method. The residual AF488 is removed by molecular sieve treatment during purification process.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

ACRO Quality Management System

1. QMS(ISO, GMP)
2. Quality Advantages
3. Quality Control Process