Human CTGF / CCN2 Protein, Fc Tag

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Cat. No. / Size
Price
Qty
GTF-H5253-100ug
$395.00
GTF-H5253-1mg (250ug X 4)
$2615.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    CCN2, NOV2, HCS24, IGFBP8, IBP-8, IGFBP-8, IGF-binding protein 8

  • Source

    Human CTGF, Fc Tag (GTF-H5253) is expressed from human 293 cells (HEK293). It contains AA Gln 27 - Ala 349 (Accession # Q5M8T4-1 ).

    Predicted N-terminus: Pro

    Request for sequence
  • Molecular Characterization

    CTGF Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the N-terminus.

    The protein has a calculated MW of 61.9 kDa. The protein migrates as 48-53 kDa and 65-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    CTGF SDS-PAGE

    Human CTGF, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • Bioactivity-ELISA

     CTGF ELISA

    Immobilized Human CTGF, Fc Tag (Cat. No. GTF-H5253) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CTGF Antibody, Human IgG1 with a linear range of 0.8-6 ng/mL (QC tested).

    Protocol

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Background

Connective Tissue Growth Factor (CTGF), also known as CCN2, is a member of the CCN (CCN1-6) family of modular matricellular proteins. Like other CCN proteins, mature human CTGF consists of IGF-binding protein domain, a vWF-C domain, a TSP-1 domain, and a cysteine knot heparin-binding domain. CTGF promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis. Analysis of CCN2 function in vivo has focused primarily on its key role as a mediator of excess ECM synthesis in multiple fibrotic diseases.

Recent Advances

 
Drug Development Progress
  • English Name:

    Connective tissue growth factor

  • Category:

  • Approved Drugs:

    1 Details

  • Drugs in Clinical Trials:

    5 Details

  • Highest Development Stage:

    Approved

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