Human DMPK Protein, His Tag (active enzyme, MALS verified)

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製品番号/仕様
価格
数量
DMK-H5143-10ug
$248.00
DMK-H5143-50ug
$968.00
DMK-H5143-500ug
$2438.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    DM, DM1, DM1PK, DMK, MDPK, MT-PK

  • Source

    Human DMPK Protein, His Tag (DMK-H5143) is expressed from E. coli cells. It contains AA Leu 10 - Pro 420 (Accession # Q09013-9).

    Predicted N-terminus: Met

    Request for sequence
  • Molecular Characterization

    DMPK Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 48.0 kDa. The protein migrates as 45-50 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

    The protein is designed as a dimer.

  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Supplied as 0.2 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping and Storage

    This product is supplied and shipped on dry ice.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    DMPK SDS-PAGE

    Human DMPK Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    DMPK SEC-MALS

    The purity of Human DMPK Protein, His Tag (Cat. No. DMK-H5143) is more than 90% and the molecular weight of this protein is around 86-100 kDa verified by SEC-MALS.

    Report
  • Bioactivity

    The DMPK assay is performed using the ADP-GloTM Kinase Assa kit which quantifies the amount of ADP produced by the DMPK reaction. The ADP-GloTM Reagent is added to terminate the kinase reaction and to deplete the remaining ATP, and then the Kinase Detection Reagent is added to convert ADP to ATP and to measure the newly synthesized ATP using luciferase/luciferin reaction. The specific activity is >2 pmol/min/ug (QC tested).

    Protocol

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バックグランド

DMPK (Dystrophia Myotonica Protein Kinase) is a serine/threonine kinase involved in cytoskeletal organization, calcium homeostasis, and muscle function. Mutations or dysregulation of DMPK are linked to myotonic dystrophy type 1 (DM1). The enzyme is widely studied as a signaling regulator and potential therapeutic target.

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