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製品詳細
Synonyms
EGF, URG, HOMG4
Source
Alexa Fluor 488-Labeled Human EGF Protein, His Tag (EGF-HA243) is produced via conjugation of AF488 to Human EGF, His Tag with a new generation site-specific technology under Star Staining labeling platform. Human EGF, His Tag is expressed from human 293 cells (HEK293). It contains AA Asn 971 - Arg 1023 (Accession # P01133-1).
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus.
Conjugate
AF488
Excitation Wavelength: 488 nm
Emission Wavelength: 517 nm
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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データ表示
Bioactivity-ELISA
Immobilized Alexa Fluor 488-Labeled Human EGF Protein, His Tag (Cat. No. EGF-HA243) at 5 μg/mL (100 μL/well) can bind Human EGF R, Fc Tag (Cat. No. EGR-H5252) with a linear range of 0.05-1 ng/mL (Routinely tested).
Protocol
Bioactivity-Flow Cytometry
2e5 of HEK293/Human EGF R Stable Cell Line (Cat. No. CHEK-ATP148) were stained with 100 μL of 0.3 μg/mL of Alexa Fluor 488-Labeled Human EGF Protein, His Tag (Cat. No. EGF-HA243) and negative control protein respectively, washed and then analyzed with FACS (QC tested).
Protocol
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よくある質問
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
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最新の進展
医薬品開発の進捗- 英語名：
Epidermal growth factor
- カテゴリ：
- 市販の医薬品数：
0 詳細
- 治験医薬品数：
4 詳細
- 最新の開発ステージ：
Phase 2 Clinical
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