Mouse EGF Protein, Fc Tag

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製品番号/仕様
価格
数量
EGF-M5265-100ug
$330.00
EGF-M5265-1mg (500ug X 2)
$2165.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    EGF, URG, HOMG4

  • Source

    Mouse EGF, Fc Tag (EGF-M5265) is expressed from human 293 cells (HEK293). It contains AA Asn 977 - Arg 1029 (Accession # P01132-1).

    Predicted N-terminus: Pro

    Request for sequence
  • Molecular Characterization

    EGF Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the N-terminus.

    The protein has a calculated MW of 32.5 kDa. The protein migrates as 35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    EGF SDS-PAGE

    Mouse EGF, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • Bioactivity-ELISA

     EGF ELISA

    Immobilized Mouse EGF R, His Tag (Cat. No. EGR-M5224) at 5 μg/mL (100 μL/well) can bind Mouse EGF, Fc Tag (Cat. No. EGF-M5265) with a linear range of 0.2-5 ng/mL (QC tested).

    Protocol
  • Bioactivity-SPR

     EGF SPR

    Captured Mouse EGF R, His Tag (Cat. No. EGR-M5224) on CM5 Chip via anti-His antibody can bind Mouse EGF, Fc Tag (Cat. No. EGF-M5265) with an affinity constant of 5.44 nM as determined in SPR assay (Biacore T200 ) (Routinely tested).

    Protocol

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バックグランド

Human epidermal growth factor (EGF) is also known as HOMG4 and URG,and is a growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR. Epidermal growth factor can be found in human platelets, macrophages, urine, saliva, milk, and plasma. EGF is the founding member of the EGF-family of proteins. Members of this protein family have highly similar structural and functional characteristics. All family members contain one or more repeats of the conserved amino acid sequence. The biological effects of salivary EGF include healing of oral and gastroesophageal ulcers, inhibition of gastric acid secretion, stimulation of D synthesis as well as mucosal protection from intraluminal injurious factors such as gastric acid, bile acids, pepsin, and trypsin and to physical, chemical and bacterial agents. Because of the increased risk of cancer by EGF, inhibiting it decreases cancer risk.

最新の進展

 
医薬品開発の進捗
  • 英語名:

    Epidermal growth factor

  • カテゴリ:

  • 市販の医薬品数:

    0 詳細

  • 治験医薬品数:

    4 詳細

  • 最新の開発ステージ:

    Phase 2 Clinical

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