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製品詳細
Synonyms
EGFR, ERBB, ERBB1, HER1, PIG61, mENA
Source
Human EGF R, His Tag (EGR-H5222) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645 (Accession # P00533-1).
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 70.5 kDa. The protein migrates as 95-110 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Mass Spectrometry
Measured Molecular Mass
The molecular weight by mass spectrometry is 70481 Da (Routinely tested).
Sequence Coverage
Human EGF R, His Tag(EGR-H5222) was digested with trypsin and characterized by LC-MS/MS peptide mapping, achieving 100% sequence coverage (Routinely tested).
N-terminal Sequence Analysis
Leu‑Glu‑Glu‑Lys‑Lys‑Val‑Cys‑Gln‑Gly‑Thr‑Ser‑Asn‑Lys‑Leu‑Thr
(Routinely tested)
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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データ表示
SDS-PAGE
Human EGF R, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human EGF R, His Tag (Cat. No. EGR-H5222) is more than 90% and the molecular weight of this protein is around 80-105 kDa verified by SEC-MALS.
Report
Mass Spectrometry
ESI‑MS analysis of Human EGF R, His Tag (Cat. No. EGR-H5222), showing a major peak at 70481 Da (Routinely tested).
Bioactivity-ELISA
Immobilized Human EGF R, His Tag (Cat. No. EGR-H5222) at 1 μg/mL (100 μL/well) can bind Anti-EGF R antibody,Human IgG1 | Human Kappa with a linear range of 0.12-1.25 ng/mL (QC tested).
Protocol
Immobilized Human EGF R, His Tag (Cat. No. EGR-H5222) at 5 μg/mL (100 μL/well) can bind Human EGF, Mouse IgG2a Fc Tag (Cat. No. EGF-H525b) with a linear range of 1-20 ng/mL (Routinely tested).
Protocol
Immobilized Human EGF R, His Tag (Cat. No. EGR-H5222) at 1 μg/mL (100 μL/well) can bind Cetuximab with a linear range of 0.1-1 ng/mL (Routinely tested).
Protocol
Immobilized Human EGF R, His Tag (Cat. No. EGR-H5222) at 1 μg/mL (100 μL/well) can bind Anti-EGFR Peptide with a linear range of 0.06-2 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Erbitux (Cetuximab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human EGF R, His Tag (Cat. No. EGR-H5222) with an affinity constant of 1.3 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Human EGF R, His Tag (Cat. No. EGR-H5222) captured on CM5 Chip via anti-His antibody can bind Human EGF, Mouse IgG2a Fc Tag (Cat. No. EGF-H525b) with an affinity constant of 2.63 nM as determined in SPR assay (Biacore T200) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Erbitux (Cetuximab) on AHC Biosensor, can bind Human EGF R, His Tag (Cat. No. EGR-H5222) with an affinity constant of 1.23 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Bioactivity-Flow Cytometry
2e5 of Anti-EGFR CAR-293 cells were stained with 100 μL of 1 μg/mL of Human EGF R, His Tag (Cat. No. EGR-H5222) and negative control protein respectively, washed and then followed by PE anti-His antibody and analyzed with FACS (Routinely tested).
Protocol
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よくある質問
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
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最新の進展
医薬品開発の進捗- 英語名：
Epidermal growth factor receptor erbB1
- カテゴリ：
- 市販の医薬品数：
39 詳細
- 治験医薬品数：
206 詳細
- 最新の開発ステージ：
Approved
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