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Human cytomegalovirus (strain Merlin) (HHV-5) Envelope protein H&L&UL128&UL130&UL131, Twin-Strep Tag&His Tag (MALS verified)

COADS MS
DS

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ENN-V5283-50ug
$925.00
In Stock - Delivery in 2-3 business days
ENN-V5283-500ug
$4740.00
In Stock - Delivery in 2 weeks
総アイテム数 製品金額$ 0
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製品詳細

  • Synonyms

    Envelope protein H&L&UL128&UL130&UL131

  • Source

    Human cytomegalovirus Envelope protein H&L&UL128&UL130&UL131, Twin-Strep Tag&His Tag (ENN-V5283) is expressed from human 293 cells (HEK293). It contains AA Ala 31 - Arg 278 & Arg 24 - Leu 719 (H&L) & Glu 28 - Gln 171 (UL128) & Ser 26 - Val 214 (UL130) & Gln 19 - Asn 129 (UL131) (Accession # Q6SW67 & F5HCH8 (H and L) & V9LLX6 (UL128) & F5HCP3 (UL130) & F5HET4 (UL131)).

    Predicted N-terminus: Trp (H&L) & Glu 28 (UL128) & Ser 26 (UL130) & Gln 19 (UL131)

    Request for sequence

  • Molecular Characterization

    This protein carries a twin strep tag and polyhistidine tag.

    The protein has a calculated MW of 110.8 kDa (H&L) & 16.7 kDa (UL128) & 23.7 kDa (UL130) & 13.0 kDa (UL131). The protein migrates as 15 kDa, 35 kDa and 130 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

    The protein is designed as a heterologous pentamer.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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  • SDS-PAGE

    Envelope protein H&L&UL128&UL130&UL131 SDS-PAGE

    Human cytomegalovirus Envelope protein H&L&UL128&UL130&UL131, Twin-Strep Tag&His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    Envelope protein H&L&UL128&UL130&UL131 SEC-MALS

    The purity of Human cytomegalovirus Envelope protein H&L&UL128&UL130&UL131, Twin-Strep Tag&His Tag (Cat. No. ENN-V5283) is more than 85% and the molecular weight of this protein is around 170-200 kDa verified by SEC-MALS.

    Report

  • Bioactivity-ELISA

    Envelope protein H&L&UL128&UL130&UL131 ELISA

    Immobilized Human Neuropilin-2, His Tag (Cat. No. NR2-H52H3) at 5 μg/mL (100 μL/well) can bind Human cytomegalovirus Envelope protein H&L&UL128&UL130&UL131, Twin-Strep Tag&His Tag (Cat. No. ENN-V5283) with a linear range of 0.004-0.125 μg/mL (QC tested).

    Protocol

  • Bioactivity-SPR

    Envelope protein H&L&UL128&UL130&UL131 SPR

    Human Neuropilin-2, His Tag (Cat. No. NR2-H52H3) immobilized on CM5 Chip can bind Human cytomegalovirus Envelope protein H&L&UL128&UL130&UL131, Twin-Strep Tag&His Tag (Cat. No. ENN-V5283) with an affinity constant of 3.41 µM as determined in a SPR assay (Biacore 8K) (Routinely tested).

    Protocol

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  • product

    Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?

    In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.


    Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.


    To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.


    To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.


    Supporting Document


バックグランド

Human cytomegalovirus (HCMV), a prototypical beta-herpes virus, is a major cause of morbidity and mortality in immunocompromised transplant recipients and congenitally infected fetuses.Different HCMV glycoprotein complexes are capable of meditating entry through interaction with cell-specific receptors on a panel of host cells. The pentamer (gH/gL/UL128/UL130/UL131A) mediates HCMV entry into epithelial, endothelial, and myeloid cells by its binding to neuropilin 2 (Nrp2). The trimer (gH/gL/gO) can mediate infection of all cell types by binding to platelet-derived growth factor-alpha (PDGFRα). Both pentamer and trimer need to interact with the glycoprotein B (gB), the fusogenic protein, to trigger the virus and host cell membrane fusion.

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