Human EpCAM / TROP1 Protein, His Tag

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製品番号/仕様
価格
数量
EPM-H5223-100ug
$320.00
EPM-H5223-1mg (500ug X 2)
$2050.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    EPCAM, TACSTD1, TROP1, CD326, DIAR5, EGP2, EGP314, EGP40, ESA, GA733-2, HNPCC8, HNPCC-8, KS1, 4, KSA, M4S1, MIC18, MK1

  • Source

    Human EpCAM, His Tag (EPM-H5223) is expressed from human 293 cells (HEK293). It contains AA Gln 24 - Lys 265 (Accession # AAH14785.1).

    Predicted N-terminus: Gln 24

    Request for sequence
  • Molecular Characterization

    EpCAM Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 28.2 kDa. The protein migrates as 31-40 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.1 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    EpCAM SDS-PAGE

    Human EpCAM, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-ELISA

     EpCAM ELISA

    Immobilized Human EpCAM, His Tag (Cat. No. EPM-H5223) at 1 μg/mL (100 μL/well) can bind Anti-EpCAM Antibody, Human IgG1 with a linear range of 0.1-2 ng/mL (QC tested).

    Protocol

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コメントを投稿

  • K***n2023-05-03
    いいね
    Overall, we were very pleased with the entire process of buying and using the product—shipping was quick and the product was packaged and stored well. The protein was used in the development of a lateral-flow immunoassay (LFA) to detect for EpCAM. Combinations of anti-EpCAM antibodies were first screened using the product, from which we were able to successfully see strong binding on spot tests in duplicate for certain antibody combination pairs. These tests allowed us to confidently move forward with printing and assembling proper LFAs.
  • J***a2021-09-17
    いいね
    We received the product with a fairly fast speed, we used this product for antibody screening and ELISA. The product is robust and good quality.

バックグランド

EpCAM is also known as CO171A, EGP, EGP40,GA7332, KSA, M4S, MIC18, MK1, TROP1, hEGP2, and is a pan-epithelial differentiation antigen that is expressed on almost all carcinomas as 17-1A(mAb) antigen. Its constitutional function is being elucidated. It is intricately linked with the Cadherin-Catenin pathway and hence the fundamental WNT pathway responsible for intracellular signaling and polarity. The epithelial cell adhesion molecule (Ep-CAM) is known to express in most epithelial malignancies and was reported as a tumor marker or a candidate of molecular targeting therapy.
Ep-CAM cross signaling with N-cadherin involves Pi3K, resulting in the abrogation of the cadherin adhesion complexes in epithelial cells was reported. And Epithelial cell adhesion molecule (Ep-CAM) recently received increased attention as a prognostic factor in breast cancer.

最新の進展

 
医薬品開発の進捗
  • 英語名:

    Epithelial cell adhesion molecule

  • カテゴリ:

  • 市販の医薬品数:

    1 詳細

  • 治験医薬品数:

    11 詳細

  • 最新の開発ステージ:

    Approved

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