Human ErbB3 / Her3 Protein, Twin-Strep Tag (MALS verified)

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製品番号/仕様
価格
数量
ER3-H5288-25ug
$270.00
ER3-H5288-250ug
$905.00
ER3-H5288-1mg (250ug X 4)
$2370.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    ERBB3, HER3, LCCS2, MDA-BF-1, MGC88033, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3, p85-sErbB3

  • Source

    Human ErbB3 Protein, Twin-Strep Tag (ER3-H5288) is expressed from human 293 cells (HEK293). It contains AA Ser 20 - Thr 643 (Accession # NP_001973.2).

    Predicted N-terminus: Ser 20

    Request for sequence
  • Molecular Characterization

    ErbB3 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a twin strep tag at the C-terminus.

    The protein has a calculated MW of 71.7 kDa. The protein migrates as 85-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    ErbB3 SDS-PAGE

    Human ErbB3 Protein, Twin-Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    ErbB3 SEC-MALS

    The purity of Human ErbB3 Protein, Twin-Strep Tag (Cat. No. ER3-H5288) is more than 90% and the molecular weight of this protein is around 85-120 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     ErbB3 ELISA

    Immobilized Human ErbB3 Protein, Twin-Strep Tag (Cat. No. ER3-H5288) at 2 μg/mL (100 μL/well) can bind Human NRG1 Beta 1, Fc Tag, premium grade (Cat. No. NR1-H5268) with a linear range of 0.5-8 ng/mL (QC tested).

    Protocol

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バックグランド

ErbB3,also known as Her3 (human epidermal growth factor receptor 3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain. It therefore can bind the ligand but cannot mediate the intracellular signal transduction through protein phosphorylation. However, it does form heterodimers with ErbB2 or other EGFR members responsible for tyrosine phosphorylation to give a receptor complex and initiate the related pathway, which lead to cell proliferation or differentiation. Overexpression of this protein has been reported in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.

最新の進展

 
医薬品開発の進捗
  • 英語名:

    Receptor tyrosine-protein kinase erbB-3

  • カテゴリ:

  • 市販の医薬品数:

    2 詳細

  • 治験医薬品数:

    31 詳細

  • 最新の開発ステージ:

    Approved

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