Human Erythropoietin / EPO Protein, premium grade

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製品番号/仕様
価格
数量
EPO-H4214-20ug
$125.00
EPO-H4214-50ug
$205.00
EPO-H4214-1mg
$1390.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    EPO, EP, MVCD2, Erythropoietin, Erythropoetin, Erthropoyetin, Hematopoietin, Hemopoietin

  • Source

    Human EPO Protein, premium grade (EPO-H4214) is expressed from human 293 cells (HEK293). It contains AA Ala 28 - Arg 193 (Accession # AAH93628.1).

    Predicted N-terminus: Ala 28

    It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.

    Request for sequence
  • Molecular Characterization

    Erythropoietin Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries no "tag".

    The protein has a calculated MW of 18.4 kDa. The protein migrates as 28-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.1 EU per μg by the LAL method / rFC method.

  • Sterility

    Negative

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    Erythropoietin SDS-PAGE

    Human EPO Protein, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-CELL BASE

     Erythropoietin CELL

    Human EPO Protein, premium grade (Cat. No. EPO-H4214) stimulates proliferation of Human TF-1 Cell. The EC50 value of this effect is 1.36-1.565 ng/mL (Routinely tested).

    Protocol
  • Bioactivity-ELISA

     Erythropoietin ELISA

    Immobilized Human EPO Protein, premium grade (Cat. No. EPO-H4214) at 10 μg/mL (100 μL/well) can bind Human Erythropoietin R Protein, Fc Tag (Cat. No. ERR-H5253) with a linear range of 0.6-10 ng/mL (QC tested).

    Protocol
  • Bioactivity-SPR

     Erythropoietin SPR

    Human Erythropoietin R Protein, His Tag (Cat. No. EPR-H52H4) immobilized on CM5 Chip can bind Human EPO Protein, premium grade (Cat. No. EPO-H4214) with an affinity constant of 0.142 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

    Protocol

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バックグランド

Human Erythropoietin (EPO) is also known as EP, erythropoetin or erthropoyetin, and is a glycoprotein hormone that controls erythropoiesis, or red blood cell production. EPO is a cytokine for erythrocyte (red blood cell) precursors in the bone marrow. is synthesized by renal peritubular cells in adults, with a small amount being produced in the liver. Regulation is believed to rely on a feed-back mechanism measuring blood oxygenation. Constitutively synthesized transcription factors for EPO, known as hypoxia-inducible factors (HIFs), are hydroxylated and proteosomally digested in the presence of oxygen. It binds to the erythropoietin receptor (EpoR) on the red cell surface and activates a JAK2 cascade. Erythropoietin has its primary effect on red blood cells by promoting red blood cell survival through protecting these cells from apoptosis. It also cooperates with various growth factors involved in the development of precursor red cells. EPO has a range of actions including vasoconstriction-dependent hypertension, stimulating angiogenesis, and inducing proliferation of smooth muscle fibers. It has also been shown that erythropoietin can increase iron absorption by suppressing the hormone hepcidin. Erythropoietin has been shown to interact with the Erythropoietin receptor as its mechanism of action within the body. erythropoietin plays an important role in the brain's response to neuronal injury. EPO is also involved in the wound healing process.

最新の進展

 
医薬品開発の進捗
  • 英語名:

    Erythropoietin

  • カテゴリ:

  • 市販の医薬品数:

    0 詳細

  • 治験医薬品数:

    1 詳細

  • 最新の開発ステージ:

    Phase 2 Clinical

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