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COADS MSDS

GMP Human FGF-8b Protein

DMF
Premium grade (Pre-GMP) version FGB-H5115 is now available for seamless transition.
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COADS MSDS
Cat. No. / Size
Price
Availability
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GMP-FGBH16-100ug
$1,295.00
In Stock - Delivery in 1-2 business days
GMP-FGBH16-1mg (100ug X 10)
$7,350.00
In Stock - Delivery in 1-2 business days
GMP-FGBH16-10mg (100ug X 100)
$51,450.00
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GMP-FGBH16
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Product Details
Performance Data
Reviews & Citations
Important Statement
Resources
  • Product Details
  • Performance Data
  • Reviews & Citations
  • Important Statement
  • Resources
Product Details

FGF8b is an isoform of Fibroblast Growth Factor 8, a key signaling molecule crucial for embryonic development, particularly in establishing the anterior-posterior axis of the brain. In neural differentiation from stem cells, it acts as a posteriorizing factor, promoting the specification of midbrain and hindbrain neuronal fates, such as dopaminergic and serotonergic neurons.

Product Features and Advantages
  • Unique Clinical-Grade Offering: The only GMP-grade FGF8b available, validated in multiple cell manufacturing processes.
  • Specific Neuronal Differentiation Driver​: Efficiently induces stem cell differentiation into neuronal progenitor cells and dopaminergic neurons.
  • Safe Recombinant Expression​: Produced in an E. coli system to ensure high purity and enhanced safety profile.
  • Cost-Effective Solution​: Achieves up to 50% cost reduction compared to conventional alternatives.
GMP Platform Advantages
  • Quality Assurance: Global QMS with comprehensive and stringent QC release criteria.
  • End-to-End GMP Compliance: Full manufacturing and QC under a cGMP system.
  • Comprehensive Control of Adventitious Agents: Stringent biosafety from cell banks to final release.
  • Comprehensive Regulatory Support: Includes RSF and DMF to meet global requirements.
  • Resilient Supply Chain: Intelligent modular facilities ensure a stable global supply.
  • Professional Support: Extensive manufacturing and application expertise to accelerate development.
Source

GMP Human FGF-8b Protein (GMP-FGBH16) is expressed from E. coli cells. It contains AA Gln 23 - Arg 215 (Accession # P55075-3).

Molecular Characterization
Other Tags and Version Biotin & Other Labeled Version

This protein carries no "tag".

The protein has a calculated MW of 22.5 kDa. The protein migrates as 25 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

N-terminal Sequence Analysis

Met-Gln-Val-Thr-Val-Gln-Ser-Ser-Pro-Asn-Phe-Thr-Gln-His-Val
(Routinely tested)

Endotoxin

Less than 10 EU/mg, tested by the LAL method in compliance with USP <85> and Ph. Eur. 2.6.14.

Host Cell Protein

<0.5 ng/µg of protein tested by ELISA.

Host Cell DNA

<0.02 ng/μg of protein tested by qPCR.

Sterility

Sterility testing was performed using the membrane filtration method in compliance with USP <71> and Ph. Eur. 2.6.1.

Mycoplasma

Negative

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with protectants.

Contact us for customized product form or formulation.

Vial Specification

2R (13 mm neck finish)

Shipping and Storage

This product is supplied and shipped on blue ice.

Upon receipt, store it immediately at -20°C or lower for long term storage.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 5 years in lyophilized state;
  • -70°C for 12 months under sterile conditions after reconstitution.
ACRO Quality Management System

Background

FGF-8 is a member of the fibroblast growth factor family that was originally discovered as a growth factor essential for the androgen-dependent growth of mouse mammary carcinoma cells (1-3). Alternate splicing of mouse FGF-8 mR generates eight secreted isoforms, designated a-h, but only FGF-8a, b, e and f exist in humans (4). FGF-8 contains a 22 amino acid (aa) signal sequence, an N‑terminal domain that varies according to the isoform (30 aa for FGF-8b; 20 aa for the shortest, FGF-8a), a 125 aa FGF domain and a 37 aa proline‑rich C‑terminal sequence. The FGF domain of FGF-8 shares the most aa identity with FGF17 (75%) and FGF-18 (67%), and the three form an FGF subfamily (2). Mouse FGF-8b shares 100% aa identity with human FGF-8b. FGF-8 is widely expressed during embryogenesis, and mediates epithelial-mesenchymal transitions. It plays an organizing and inducing role during gastrulation, and regulates patterning of the midbrain/hindbrain, eye, ear, limbs and heart in the embryo (2, 5 - 8). The isoforms may play different roles in development. FGF-8b shows the strongest receptor affinity and oncogenic transforming capacity although FGF-8a and FGF-8e are also transforming and have been found in human prostate, breast or ovarian tumors (1, 5, 9-12). FGF-8 shows limited expression in the normal adult, but low levels are found in the reproductive and genitourinary tract, peripheral leukocytes and bone marrow hematopoietic cells (3, 9, 13).