Mouse Flt-3 / Flk-2 Protein, His Tag

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製品番号/仕様
価格
数量
FL3-M52H6-100ug
$360.00
FL3-M52H6-1mg (200ug X 5)
$2615.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    Flt-3, Flk-2, STK-1, CD135, FLK2, FLT-3

  • Source

    Mouse Flt-3, His Tag (FL3-M52H6) is expressed from human 293 cells (HEK293). It contains AA Asn 28 - Ser 544 (Accession # NP_034359.2).

    Predicted N-terminus: Asn 28

    Request for sequence
  • Molecular Characterization

    Flt-3 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 59.9 kDa. The protein migrates as 70-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    Flt-3 SDS-PAGE

    Mouse Flt-3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • Bioactivity-ELISA

     Flt-3 ELISA

    Immobilized Mouse Flt-3, His Tag (Cat. No. FL3-M52H6) at 5 μg/mL (100 μL/well) can bind CD135 (FLT3) Antibody, Anti-mouse with a linear range of 0.02-0.625 μg/mL (Routinely tested).

    Protocol
  • Bioactivity-SPR

     Flt-3 SPR

    Mouse Flt-3, His Tag (Cat. No. FL3-M52H6) immobilized on CM5 Chip can bind Mouse Flt-3 Ligand, His Tag (Cat. No. FLL-M52H4) with an affinity constant of 59.6 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

    Protocol

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バックグランド

Flt-3(Receptor-type tyrosine-protein kinase FLT3) is also known as FLK-2(Fetal liver kinase-2), STK-1(Stem cell tyrosine kinase 1), CD135. FLT3 is a cytokine receptor which belongs to the receptor tyrosine kinase class III. Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways.

最新の進展

 
医薬品開発の進捗
  • 英語名:

    Tyrosine-protein kinase receptor FLT3

  • カテゴリ:

  • 市販の医薬品数:

    14 詳細

  • 治験医薬品数:

    59 詳細

  • 最新の開発ステージ:

    Approved

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