Human FoxP3 Protein, His Tag

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Cat. No. / Size
Price
Qty
FO3-H5243-100ug
$360.00
FO3-H5243-1mg (200ug X 5)
$2380.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    Forkhead box protein P3, FOXP3, IPEX, JM2, AIID, PIDX, XPID, DIETER

  • Source

    Human FoxP3 Protein, His Tag (FO3-H5243) is expressed from E. coli cells. It contains AA Met 1 - Pro 431 (Accession # Q9BZS1-1).

    Predicted N-terminus: His

    Request for sequence
  • Molecular Characterization

    FoxP3 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 49.1 kDa. The protein migrates as 51-53 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 0.5 M Arginine, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    FoxP3 SDS-PAGE

    Human FoxP3 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-ELISA

     FoxP3 ELISA

    Immobilized Human FoxP3 Protein, His Tag (Cat. No. FO3-H5243) at 1 μg/mL (100 μL/well) can bind Anti-FOXP3 antibody, Mouse IgG1 with a linear range of 0.5-8 ng/mL (QC tested).

    Protocol

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Background

FOXP3 is a transcriptional regulator which is crucial for the development and inhibitory function of regulatory T-cells (Treg). It plays an essential role in maintaining homeostasis of the immune system by allowing the acquisition of full suppressive function and stability of the Treg lineage, and by directly modulating the expansion and function of conventional T-cells. Can act either as a transcriptional repressor or a transcriptional activator depending on its interactions with other transcription factors, histone acetylases and deacetylases. The suppressive activity of Treg involves the coordinate activation of many genes, including CTLA4 and TNFRSF18 by FOXP3 along with repression of genes encoding cytokines such as interleukin-2 (IL2) and interferon-gamma (IFNG). Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome.

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