Product Details
Synonyms
Glycoprotein E2 (RUBV)
Source
Rubella virus Glycoprotein E2 (strain M33) (RUBV), His Tag (GL2-R52H3) is expressed from human 293 cells (HEK293). It contains AA Gly 301- Ala 532 (Accession # P08563).
Predicted N-terminus: Gly 301
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 26.7 kDa. The protein migrates as 45-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE

Rubella virus Glycoprotein E2 (strain M33) (RUBV), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS

The purity of Rubella virus Glycoprotein E2 (strain M33) (RUBV), His Tag (Cat. No. GL2-R52H3) is more than 85% and the molecular weight of this protein is around 53-65 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA

Immobilized Rubella virus Glycoprotein E2 (strain M33) (RUBV), His Tag (Cat. No. GL2-R52H3) at 1 μg/mL (100 μL/well) can bind Antibodies Rubella Virus E2 Antibody, Mouse IgG with a linear range of 0.1-8 ng/mL (QC tested).
Protocol
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