Human GMF-beta Protein, His Tag, low endotoxin (MALS verified)

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GMA-H5145-20ug
$105.00
GMA-H5145-50ug
$185.00
GMA-H5145-500ug
$1,110.00
GMA-H5145
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  • Product Details
  • Performance Data
  • Reviews & Citations
  • Important Statement
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  • 分子别名(Synonym)

    GMF-beta,Glia maturation factor beta,GMFB

  • 表达区间及表达系统(Source)

    Human GMF-beta, His Tag (GMA-H5145) is expressed from E. coli cells. It contains AA Ser 2 - His 142 (Accession # P60983).

    Predicted N-terminus: His

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    GMF-beta Structure

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 18.6 kDa. The protein migrates as 19-21 kDa under reducing (R) condition (SDS-PAGE).

  • 内毒素(Endotoxin)

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • 无菌(Sterility)

    Negative

  • 支原体(Mycoplasma)

    Negative.

  • 纯度(Purity)

    >95% as determined by SDS-PAGE.

    >98% as determined by SEC-MALS.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

Background

Glia Maturation Factor-Beta (GMF-Beta) is a 17 kDa protein nerve gorwth factor identified as a growth and differentiation factor in the vertebrate brain.
Glia Maturation Factor-Beta stimulates differentiation of normal neurons as well as glial cells. GMFB inhibits the proliferation of the N-18 neuroblastoma line and the C6 glioma line while promoting their phenotypic expression.
GMF-beta inhances the phenotypic expression of glia & neurons thus inhibits the proliferation of their respective tumors when added to cell culture. Although astrocytes produce GMF-b and stores it inside the cells, they don’t secrete the GMF-B into the cultured medium. Cell- surface GMFb acts on the target cells at close range when cells are in direct contact. GMF-Beta is produced by thymic epithelial cells and plays an important role in T cell development in favor of CD4+ T cells.
GMF-Beta is a brain-specific protein which belongs to the actin-binding proteins (ADF) family. GMF-beta appears to play a role in the differentiation, maintenance, and regeneration of the nervous system. It also supports the progression of certain auto-immune diseases, possibly through its ability to induce the production and secretion of various pro-inflammatory cytokines.