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製品詳細
Synonyms
ERBB2, CD340, HER-2, neu, HER2, MLN19, NEU, NGL, TKR1
Source
Human Her2, Tag Free (HE2-H5212) is expressed from human 293 cells (HEK293). It contains AA Thr 23 - Thr 652 (Accession # P04626-1).
Predicted N-terminus: Thr 23
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries no "tag".
The protein has a calculated MW of 70.1 kDa. The protein migrates as 90-100 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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データ表示
SDS-PAGE
Human Her2, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human Her2, Tag Free (Cat. No. HE2-H5212) is more than 85% and the molecular weight of this protein is around 75-110 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human Her2, Tag Free (Cat. No. HE2-H5212) at 1 μg/mL (100 μL/well) can bind Trastuzumab Biosimilar with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
Immobilized Human Her2, Tag Free (Cat. No. HE2-H5212) at 1 μg/mL (100 μL/well) can bind Bispecific Antibody (Her2 × Her3) with a linear range of 0.1-2 ng/mL (Routinely tested).
Protocol
Immobilized Human Her2, Tag Free (Cat. No. HE2-H5212) at 1 μg/mL (100 μL/well) can bind Pertuzumab Biosimilar with a linear range of 0.1-4 ng/mL (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Hercecptin on AHC Biosensor, can bind Human Her2, Tag Free (Cat. No. HE2-H5212) with an affinity constant of 1.45 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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最新の進展
医薬品開発の進捗- 英語名：
Receptor protein-tyrosine kinase erbB-2
- カテゴリ：
- 市販の医薬品数：
47 詳細
- 治験医薬品数：
238 詳細
- 最新の開発ステージ：
Approved
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