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Product Details
Synonyms
ERBB2, CD340, HER-2, neu, HER2, MLN19, NEU, NGL, TKR1
Source
Human Her2 (498-648), His Tag (HE2-H52H4) is expressed from human 293 cells (HEK293). It contains AA Asn 498 - Ala 648 (Accession # P04626-1).
Predicted N-terminus: Asn 498
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 18.5 kDa. The protein migrates as 28-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. This protein has been verified not recognized by Pertuzumab in functional ELISA at ACRObiosystems.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Human Her2 (498-648), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human Her2 (498-648), His Tag (Cat. No. HE2-H52H4) is more than 85% and the molecular weight of this protein is around 22-40 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human Her2 (498-648), His Tag (Cat. No. HE2-H52H4) at 1 μg/mL (100 μL/well) can bind Trastuzumab Biosimilar with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
Immobilized Human Her2 (498-648), His Tag (Cat. No. HE2-H52H4) at 2 μg/mL (100 μL/well) can bind Trastuzumab with a linear range of 0.2-1 ng/mL, This protein has been shown to weakly bind to Pertuzumab in our functional ELISA studies.(Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Receptor protein-tyrosine kinase erbB-2
- Category：
- Approved Drugs：
47 Details
- Drugs in Clinical Trials：
238 Details
- Highest Development Stage：
Approved
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