Alexa Fluor™ 488-Labeled Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer Protein (MALS verified)

Synonyms

HLA-A*0201 & B2M & WT-1 (RMFPNAPYL)

Source

Alexa Fluor 488-Labeled Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer Protein (HLW-HA2H5) is expressed from human 293 cells (HEK293).

Predicted N-terminus: Gly 25 & Ile 21

Molecular Characterization

Alexa Fluor 488-Labeled Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer Protein is assembled by biotinylated monomer (HLW-H82E5) and AF488-labeled streptavidin.

Biotinylated Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Complex Protein is produced by co-expression of HLA and B2M loaded with WT-1 peptide. Biotinylated Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Complex Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 36.3 kDa and 11.7 kDa. The protein migrates as 40-43 kDa and 13 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Conjugate

AF488

Excitation Wavelength: 488 nm

Emission Wavelength: 517 nm

Labeling

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with AF488 using standard chemical labeling method. The residual AF488 is removed by molecular sieve treatment during purification process.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

ACRO Quality Management System

1. QMS(ISO, GMP)
2. Quality Advantages
3. Quality Control Process