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IG1-H82E2-50ug
$448.00
IG1-H82E2-25ug
$360.00
IG1-H82E2-500ug (250ug × 2)
$1,750.00
IG1-H82E2
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分子别名(Synonym)

IgG1

表达区间及表达系统(Source)

MABSol® Biotinylated Human IgG1 Fc, Avitag,His Tag (IG1-H82E2) is expressed from human HEK293 cells. It contains AA Pro 100 - Lys 330 (Accession # P01857-1 (C103S)).

Predicted N-terminus: N/A

Request for sequence

蛋白结构(Molecular Characterization)

IgG1 Fc Structure

This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag

The protein has a calculated MW of 28.8 kDa. The protein migrates as 33-38 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 12 months under sterile conditions after reconstitution.

Background

Crystallizable fragments composed of the carboxy-terminal halves of both IMMUNOGLOBULIN HEAVY CHAINS linked to each other by disulfide bonds. Fc fragments contain the carboxy-terminal parts of the heavy chain constant regions that are responsible for the effector functions of an immunoglobulin (COMPLEMENT fixation, binding to the cell membrane via FC RECEPTORS, and placental transport). IgG1 Fc was reported has a novel role as a potential anti-inflammatory drug for treatment of human autoimmune diseases.