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Product Details
Synonyms
IL-11 & IL-11 R alpha
Source
Human IL-11 & IL-11 R alpha Protein, His Tag (IL1-H52H6) is expressed from human 293 cells (HEK293). It contains AA Ser 24 - Ala 370 & Pro 22 - Leu 199 (Accession # P20809-1 & Q14626-1).
Predicted N-terminus: Ser 24
Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 60 kDa. The protein migrates as 58-68 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Human IL-11 & IL-11 R alpha Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human IL-11 & IL-11 R alpha Protein, His Tag (Cat. No. IL1-H52H6) is more than 85% and the molecular weight of this protein is around 60-80 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human gp130 Protein, Fc Tag (Cat. No. ILT-H5254) at 5 μg/mL (100 μL/well) can bind Human IL-11 & IL-11 R alpha Protein, His Tag (Cat. No. IL1-H52H6) with a linear range of 0.3-78 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Human gp130, His Tag (Cat. No. ILT-H52H2) immobilized on CM5 Chip can bind Human IL-11 & IL-11 R alpha Protein, His Tag (Cat. No. IL1-H52H6) with an affinity constant of 18.9 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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