Product Details
Synonyms
IL-12 R beta 2, IL12RB2, IL-12RB2, IL-12R-beta-2
Source
Mouse IL-12 R beta 2 Protein, His Tag (ILB-M52H3) is expressed from human 293 cells (HEK293). It contains AA Asn 24 - Asn 637 (Accession # P97378).
Predicted N-terminus: Asn 24
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 70.5 kDa. The protein migrates as 95-130 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE

Mouse IL-12 R beta 2 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-SPR

Mouse IL-12B&IL-12A Heterodimer Protein, Tag Free&His Tag (Cat. No. IL2-M52W3) immobilized on CM5 Chip can bind Mouse IL-12 R beta 2 Protein, His Tag (Cat. No. ILB-M52H3) with an affinity constant of 971 nM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
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