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Product Details
Synonyms
IL-17 RC, IL-17RC, IL17Rhom, IL-17 receptor C, IL-17RL, ZcytoR14
Source
Human IL-17 RC (21-465) Protein, Fc Tag (ILC-H5258) is expressed from human 293 cells (HEK293). It contains AA Leu 21 - His 465 (Accession # NP_703190.2).
Predicted N-terminus: Leu 21
Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 76 kDa. The protein migrates as 100-130 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human IL-17 RC (21-465) Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human IL-17 RC (21-465) Protein, Fc Tag (Cat. No. ILC-H5258) is more than 95% and the molecular weight of this protein is around 95-130 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human IL17A, premium grade (Cat. No. ILA-H5219) at 2 μg/mL (100 μL/well) can bind Human IL-17 RC (21-465) Protein, Fc Tag (Cat. No. ILC-H5258) with a linear range of 2-78 ng/mL (QC tested).
Protocol
Immobilized Human IL-17F (H161R), His Tag (Cat. No. ILF-H4240) at 2 μg/mL (100 μL/well) can bind Human IL-17 RC (21-465) Protein, Fc Tag (Cat. No. ILC-H5258) with a linear range of 0.02-0.625 μg/mL (Routinely tested).
Protocol
Bioactivity-SPR
Human IL-17 RC (21-465) Protein, Fc Tag (Cat. No. ILC-H5258) immobilized on CM5 Chip can bind Human IL17A, premium grade (Cat. No. ILA-H5219) with an affinity constant of 0.718 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Human IL-17 RC (21-465) Protein, Fc Tag (Cat. No. ILC-H5258) immobilized on CM5 Chip can bind Biotinylated Human IL17A, His,Avitag (Cat. No. ILA-H82Q1) with an affinity constant of 2.24 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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