Rhesus macaque IL-18 Protein, Tag Free (MALS & SPR verified)

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Cat. No. / Size
Price
Qty
IL8-R5113-100ug
$350.00
IL8-R5113-1mg (200ug X 5)
$2310.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    IL-18, Interleukin-18, IL18, IL-1 gamma, IGIF, IL1F4, IL-1F4

  • Source

    Rhesus macaque IL-18 Protein, Tag Free (IL8-R5113) is expressed from E. coli cells. It contains AA Try 37 - Asp 193 (Accession # NP_001028006.1).

    Predicted N-terminus: Try 37

    Request for sequence
  • Molecular Characterization

    IL-18 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries no "tag".

    The protein has a calculated MW of 18.3 kDa. The protein migrates as 15-17 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    IL-18 SDS-PAGE

    Rhesus macaque IL-18 Protein, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    IL-18 SEC-MALS

    The purity of Rhesus macaque IL-18 Protein, Tag Free (Cat. No. IL8-R5113) is more than 90% and the molecular weight of this protein is around 19-35 kDa verified by SEC-MALS.

    Report
  • Bioactivity-SPR

     IL-18 SPR

    Human IL-18 R1, Fc Tag (Cat. No. IL1-H5259) captured on Protein A Chip can bind Rhesus macaque IL-18 Protein, Tag Free (Cat. No. IL8-R5113) with an affinity constant of 4.09 nM as determined in a SPR assay (Biacore 8K) (QC tested).

    Protocol

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Background

Interleukin-18 (IL-18) is a potent proinflammatory cytokine that induces interferon-gamma (IFN-gamma) production from Th1 cells, NK cells and activated macrophages, particularly in the presence of IL-12. IL-18 also functions in developmental regulation of T-lymphocyte helper type I cells and in Fas-mediated cytotoxicity. Suppression of IL-18 activity is being investigated for treatment of chronic inflammatory diseases such as Crohn's disease and rheumatoid arthritis. It acts by inducing heterodimerization of the two subunits of its receptor, IL-18RAlpha and IL-18RBeta shows structural similarity to IL-1.

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