Human IL-27 Ra / TCCR Protein, His Tag (MALS verified)

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Cat. No. / Size
Price
Qty
ILA-H52Hb-100ug
$355.00
ILA-H52Hb-1mg (500ug X 2)
$2260.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    IL27RA, CRL1, TCCR, WSX1

  • Source

    Human IL-27 Ra Protein, His Tag (ILA-H52Hb) is expressed from human 293 cells (HEK293). It contains AA Gln 33 - Arg 514 (Accession # AAH28003.1).

    Predicted N-terminus: Gln 33

    Request for sequence
  • Molecular Characterization

    IL-27 Ra Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 54.2 kDa. The protein migrates as 70-85 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    IL-27 Ra SDS-PAGE

    Human IL-27 Ra Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    IL-27 Ra SEC-MALS

    The purity of Human IL-27 Ra Protein, His Tag (Cat. No. ILA-H52Hb) is more than 85% and the molecular weight of this protein is around 65-85 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     IL-27 Ra ELISA

    Immobilized Human IL-27 Protein, Fc Tag, premium grade (Cat. No. IL7-H5254) at 5 μg/mL (100 μL/well) can bind Human IL-27 Ra Protein, His Tag (Cat. No. ILA-H52Hb) with a linear range of 0.008-0.25 μg/mL (QC tested).

    Protocol

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Background

Interleukin-27 receptor subunit alpha ( IL27RA) is also known as Cytokine receptor WSX-1, Cytokine receptor-like 1, Type I T-cell cytokine receptor (TCCR), ZcytoR1, IL27RA belongs to the type I cytokine receptor family and type 2 subfamily. IL27RA contains three fibronectin type-III domains. IL27RA is highly expressed in lymphoid tissues such as spleen, lymph nodes and peripheral blood leukocytes. IL27RA is receptor for IL27, but requires IL6ST/gp130 to mediate signal transduction in response to IL27. IL27RA involved in the regulation of Th1-type immune responses. IL27RA also appears to be involved in innate defense mechanisms.

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