Product Details
Synonyms
IL-36Ra, FIL1 delta, IL-1RP3, IL-1HY1, IL-1 delta, IL-1F5, IL-1L1, IL1F5, IL36Ra, IL36RN
Source
Human IL-36Ra Protein, Tag Free (ILA-H5117) is expressed from E. coli cells. It contains AA Val 2 - Asp 155 (Accession # Q9UBH0).
Predicted N-terminus: Val
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries no "tag".
The protein has a calculated MW of 16.8 kDa. The protein migrates as 15-16 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE

Human IL-36Ra Protein, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-HPLC

The purity of Human IL-36Ra Protein, Tag Free (Cat. No. ILA-H5117) was greater than 90% as determined by SEC-HPLC.
Bioactivity-SPR

Biotinylated Human IL-1 Rrp2, His,Avitag (Cat. No. IL2-H82E9) immobilized on SA Chip can bind Human IL-36Ra Protein, Tag Free (Cat. No. ILA-H5117) with an affinity constant of 2.32 nM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
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