Rabbit IL-4 Protein, His Tag (MALS & SPR verified)

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Cat. No. / Size
Price
Qty
IL4-R52H3-100ug
$360.00
IL4-R52H3-1mg (500ug X 2)
$2615.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    IL4, BCGF1, BSF1

  • Source

    Rabbit IL-4 Protein, His Tag (IL4-R52H3) is expressed from human 293 cells (HEK293). It contains AA Arg 25 - Ser 147 (Accession # Q9MZR8).

    Predicted N-terminus: Arg 25

    Request for sequence
  • Molecular Characterization

    IL-4 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 16.0 kDa. The protein migrates as 20-25 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    IL-4 SDS-PAGE

    Rabbit IL-4 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    IL-4 SEC-MALS

    The purity of Rabbit IL-4 Protein, His Tag (Cat. No. IL4-R52H3) is more than 95% and the molecular weight of this protein is around 16-25 kDa verified by SEC-MALS.

    Report
  • Bioactivity-SPR

     IL-4 SPR

    Rabbit IL-4 R alpha Protein, His Tag (Cat. No. ILR-R52H8) immobilized on CM5 Chip can bind Rabbit IL-4 Protein, His Tag (Cat. No. IL4-R52H3) with an affinity constant of 0.0287 nM as determined in a SPR assay (Biacore 8K) (QC tested).

    Protocol

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Background

Interleukin-4, is a cytokine that induces differentiation of naive helper T cells (Th0 cells to Th2 cells). In the presence of IL-4 and IL-13, cytokines that are produced in a Th-2 type response, particularly during allergy and parasitic infections, macrophages become differentially activated, And this cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. Recently, researcher found that the cytokine IL-4 plays a key role in development of innate CD8+ T cells in the thymus of several gene-deficient mouse strains, including Itk, KLF2, CBP and Id3, without previous exposure to antigen.

Recent Advances

 
Drug Development Progress
  • English Name:

    Interleukin-4

  • Category:

  • Approved Drugs:

    2 Details

  • Drugs in Clinical Trials:

    14 Details

  • Highest Development Stage:

    Approved

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